ABSTRACT. Genetic parameters and breeding values for growth traits were estimated in the first and, currently, the only family selective breeding program for rainbow trout (Oncorhynchus mykiss) in China. Genetic and phenotypic data were collected for growth traits from 75 full-sibling families with a 2-generation pedigree. Genetic parameters and breeding values for growth traits of rainbow trout were estimated using the derivative-free restricted maximum likelihood method. The goodness-of-fit of the models was tested using Akaike and Bayesian information criteria. Genetic parameters and breeding values were estimated using the best-fit model for each trait. The values for heritability estimating body weight and length ranged from 0.20 to 0.45 and from 0.27 to 0.60, respectively, and the heritability of condition factor was 0.34. Our results showed a moderate degree of heritability for growth traits in this breeding program and suggested that the genetic and phenotypic tendency of body length, body weight, and condition factor were similar. Therefore, the selection of phenotypic values based on pedigree information was also suitable in this research population.

Excessive accumulation of carcass fat in farm animals, including fish, has a significant impact on meat quality and on the cost of feeding. Similar to farmed animals and humans, the liver can be considered one of the most important organs involved in lipid metabolism in rainbow trout (Oncorhynchus mykiss). RNA-seq based whole transcriptome sequencing was performed to liver tissue of rainbow trout with high and low carcass fat content in this study. In total 1,694 differentially expressed transcripts were identified, including many genes involved in lipid metabolism, such as L-FABP, adiponectin, PPAR-α, PPAR-β, and IGFBP1a. Evidence presented in this study indicated that lipid metabolic process in liver may be related to the difference of carcass fat content. The relevance of PPAR-α and PPAR-β as molecular markers for fat storage in liver should be worthy of further investigation.

Various salmonid species are cultivated in cold water aquaculture. However, due to limited genomic data resources, specific high-throughput genotyping tools are not available to many of the salmonid species. In this study, a 57K single nucleotide polymorphism (SNP) array for rainbow trout (Oncorhynchus mykiss) was utilized to detect polymorphisms in seven salmonid species, including Hucho taimen, Oncorhynchus masou, Salvelinus fontinalis, Brachymystax lenok, Salvelinus leucomaenis, O. kisutch, and O. mykiss. The number of polymorphic markers per population ranged from 3,844 (O. kisutch) to 53,734 (O. mykiss), indicating that the rainbow trout SNP array was applicable as a universal genotyping tool for other salmonid species. Among the six other salmonid populations from four genera, 28,882 SNPs were shared, whereas 525 SNPs were polymorphic in all four genera. The genetic diversity and population relationships of the seven salmonid species were studied by principal component analysis (PCA). The phylogenetic relationships among populations were analyzed using the maximum likelihood method, which indicated that the shared SNP markers provide reliable genomic information for population genetic analyses in common aquaculture salmonid fishes. Furthermore, this obtained genomic information may be applicable for population genetic evaluation, marker-assisted breeding, and propagative parent selection in fry production.

This study aims to clarify if apigenin (AP) could play a pivotal role in attenuating acrylonitrile (ACN)-induced sperm and testis injury by inhibiting ASK1-JNK/p38 signaling pathway. Male Sprague–Dawley rats were randomly divided into five groups: a control group (corn oil), an ACN group (ACN 46 mg kg−1), an ACN + AP1 group (ACN + AP 117 mg kg−1), an ACN + AP2 group (ACN + AP 234 mg kg−1) and an ACN + AP3 group (ACN + AP 351 mg kg−1). The ACN + AP groups were given ACN by gavage after a pretreatment with different dosages of AP for 30 min, whereas the rats in the control group received an equivalent volume of corn oil. The gavage was conducted for 6 days per week in 4 weeks. The results showed that AP reduced sperm deformity rate and DNA fragment index and attenuated the testicular injury induced by ACN. AP could also alleviate oxidative stress, downregulate ASK1-JNK/p38 signaling pathway and eventually inhibit mitochondria-mediated testicular apoptosis. In brief, AP could dampen oxidative stress thereby inhibiting testicular apoptosis mediated by ASK1-JNK/p38 signaling pathway, alleviating ACN-induced sperm and testis injury and exerting a protective effect on male reproductive system.

A study was conducted to compare growth and nutrients in the muscle of diploid and triploid masu salmon (Oncorhynchus masou B., 1856). Triploidy was induced by providing a heat shock treatment to the fertilized eggs. When fish grew up to 100 g around, triploids were prepared after triploidy determination prior to the trial. Initial mean weights of diploids and triploids were 103.67 ± 9.66 g and 109.95 ± 8.45 g, respectively. Quadruplicate groups of 30 fish were randomly assigned in each tank fed by water with a flow-through system and were fed to apparent satiation for 140 days. Survival, specific growth rate, feed conversion rate, condition factor, relative growth rate and absolute growth rate were determined in both groups. The contents of moisture, crude protein, lipid, ash, amino acids and fatty acids in muscle were analyzed. It has been determined that the triploid fish showed a higher weight gain than diploid fish despite the difference was not significant (P>0.05). There were no significant differences in crude protein, crude lipid, ash, amino acids and fatty acids between diploids and triploids (P>0.05). In conclusion, growth performances and nutritional composition in muscle were similar between diploids and triploids, and triploids had no negative effects on muscle nutrients under fish farming practices especially for the production of Oncorhynchus masou on-growing stages.