The determination of the enantiomeric excess and absolute configuration of chiral compounds is indispensable in synthetic, pharmaceutical, and biological chemistry. In this article, we describe an efficient 19 F nuclear magnetic resonance (NMR)based analytical protocol for determining the enantiomeric excess and absolute configuration of in situ fluorine-labeled amines and alcohols. 2-Fluorobenzoylation was used to convert analytes to fluorinated amides or esters. The resulting F-labeled analytes were mixed with a cationic cobalt(III) complex, [Co]BArF, resulting in clean baseline peak separations of analyte enantiomers in 19 F{ 1 H} NMR spectra. The measured Δδ RS signs were unambiguously used to correlate the absolute configurations of amines, amino alcohols, and alcohols. Moreover, the structure-dependent 19 F{ 1 H} NMR signals enabled absolute configuration determination by analyzing the relative chemical shifts of enantiopure analyte samples with [Co]BArF and ent-[Co]BArF.

We present a novel approach utilizing 19F-nuclear magnetic resonance (NMR) spectroscopy for serum amine profiling. Our method introduces a highly efficient and reliable technique for fluorine labeling of amine metabolites via Schiff base formation. By employing this fluorine labeling, we successfully achieve accurate identification and quantification of amine metabolites in human serums, providing valuable insights for metabolomics research.