Layout is an important factor of agro-industry to improve productivity and reduce potential risk of work accident. The objective of this research was to redesign the layout of Tempe Chips Small and Medium Enterprise (SME). Bu Nurjanah Tempe Chips SME Malang, East Java was selected as research location because of poor lay out system and it has same character with Tempe Chips SME that had fire accident. This research used Occupational Health and Safety Analysis and Systematic Layout Planning (SLP) approach in designing agro-industry layout that consist of activity relationship chart, activity relationship diagram, and area needs. The results of the research showed that Tempe Chips SME has potential fire hazards and food contamination. Regarding the Occupational Safety and Health standard, this research advice re-layout for several production facilities such as access to drinking water, toilets and washing facilities, and clean cafeteria to improve effectiveness of production.