The methanol extract of Alternanthera brasiliana Kuntze (Family: Amaranthaceae) leaf was investigated for its wound healing effect by excision wound model (in vivo) in aged Sprague Dawley rats. In excision wound model, compared to the control group, percent contraction of wound was signi icantly (P < 0.01) higher in A. brasilianatreated group (5% w/w ointment). The collagen, elastin, and hydroxyproline contents of the granulation tissue of A. brasiliana-treated group increased signi icantly (P < 0.01) compared to the control group, indicating better wound healing activity of the test plant. These indings were also con irmed by histopathological examination. The results suggested that methanol extract of A. brasiliana possesses signi icant wound healing potential in aged animal wound model.

Ocimum sanctum also known as Tulsi orHolybasil is an aromatic plant and it belongs to the family Lamiaceae. It is widely used as medicine to cure various ailments. The objective of the study was to analyse different phytochemical components of tulsi leaf. The dried powder of Tulsi (50g) was placed in the thimble of Soxhlet apparatus and the experiment was done separately for methanol, ethanol and distilled water. The percentage yield was 8%w/w,7%w/w, and 5%w/w respectively. The study reveals that various secondary metabolites such as carbohydrate, tannin, flavonoids, saponins, glycoside, terpenoid, fatty acids and phenol are present in tulsi leaf extract. From the quantitative analysis it was found that high amount of phenols are present in Tulsi leaf ranging from 1.6 to 7.6 percentages. Consequently the amount of alkaloid and flavonoids ranged from 0.91 to 1.28 and 1.56 to 2.24 percentages respectively. From the GC-MS analysis of methanolic extract three compounds were identified as major constituents viz., Eugenol , Benzene,1,1,2, analgesic, antimicrobial, antistress, immunomodulatory, hypoglycemic, hypotensive and antioxidant properties. Hence it is more beneficial to use tulsi asan herbal medicine as compare to chemically synthesized drug.

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Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.

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