Carbon isotope discrimination (Δ) and nitrogen isotope ratios, N-concentrations and specific leaf area of 50 tree species were investigated along a continental-scale transect through northern Australia over which annual rainfall varied from 1800 mm to 216 mm rainfall. Average specific leaf area (SLA, m2 kg-1) of leaves ranged from 10.7 ± 1.7 (av. ± s.d.) in N2 fixing deciduous trees to 0.8 ± 0.4 in spinescent sclerophylls shrubs. SLA generally decreased with increasing aridity. N2 fixing species had higher leaf N concentration (average N-concentration 20.1 ± 3.7 mgN g-1) than non- N2 fixing (10.8 ± 3.3) or spinescent species (7.05 ± 1.8). Community-averaged Δ was approximately constant at rainfalls above 475 mm (average Δ = 19.4 ± 1.2‰). Where rainfall was less than 475 mm, Δ decreased from 19‰ to 17‰ at 220 mm. Δ was positively correlated with SLA. Δ of deciduous N2 fixing species and spinescent species were 1‰ and 2.4‰ lower than in evergreen sclerophyllous species. Δ in the N2 fixing Allocasuarina was 1.2‰ lower than in non- N2 fixing sclerophyllous species. The δ15N-values indicated N2 fixation only at high rainfall. Burning of the field layer in a Eucalyptus forest had no effect on all measured tree parameters including δ15N, but δ15N increased under grazing conditions to >5‰. The constant value of community averaged Δ between 1800 and 450 mm may be the result of replacement of functional types and species. The decline in Δ in the more arid section may be a function of both low species diversity, and a highly aseasonal and unpredictable rainfall regime.

Two pregnancy-specific proteins were detected by immunoelectrophoresis using antisera developed to homogenates of bovine extraembryonic membranes. Antisera also reacted to extracts of endometrium from pregnant cows and extraembryonic fluids. However, antisera did not react with a preparation presumed to be bovine placental lactogen, fetuin, extracts of various somatic tissues from pregnant cows or extracts of endometrium from nonpregnant cows. One of the proteins had an estimated molecular weight of 65,000-70,000, an isoelectric point of 4.6-4.8 and yielded a reaction of identity with bovine alpha 1-fetoprotein by immunodiffusion. The second protein yielded a reaction of identity with bovine alpha 1-fetoprotein by immunodiffusion. The second protein had no immunological cross-reactivity with the known proteins or organ extracts which were tested. The molecular weight and isoelectric point was 47,000-53,000 and 4.0-4.4, respectively. These data demonstrate the presence of at least 2 pregnancy-specific proteins in cattle.

Moraxella sp. isolated from soil grows anaerobically on benzoate by nitrate respiration; nitrate or nitrite are obligatory electron acceptors, being reduced to molecular N2 during the catabolism of the substrate. This bacterium also grows aerobically on benzoate. 2. Aerobically, benzoate is metabolized by ortho cleavage of catechol followed by the beta-oxoadipate pathway. 3. Cells of Moraxella grown anaerobically on benzoate are devoid of ortho and meta cleavage enzymes; cyclohexanecarboxylate and 2-hydroxycyclohexanecarboxylate were detected in the anaerobic culture fluid. 4. [ring-U-14C]Benzoate, incubated anaerobically with cells in nitrate-phosphate buffer, gave rise to labelled 2-hydroxycyclohexanecarboxylate and adipate. When [carboxy-14C]benzoate was used, 2-hydroxycyclohexanecarboxylate was radioactive but the adipate was not labelled. A decarboxylation reaction intervenes at some stage between these two metabolites. 5. The anaerobic metabolism of benzoate by Moraxella sp. through nitrate respiration takes place by the reductive pathway (Dutton & Evans, 1969). Hydrogenation of the aromatic ring probably occurs via cyclohexa-2,5-dienecarboxylate and cyclohex-1-enecarboxylate to give cyclohexanecarboxylate. The biochemistry of this reductive process remains unclear. 6. CoA thiol esterification of cyclohexanecarboxylate followed by beta-oxidation via the unsaturated and hydroxy esters, would afford 2-oxocyclohexanecarboxylate. Subsequent events in the Moraxella culture differ from those occurring with Rhodopseudomonas palustris; decarboxylation precedes hydrolytic cleavage of the alicyclic ring to produce adipate in the former, whereas in the latter the keto ester undergoes direct hydrolytic fission to pimelate.

A study was conducted to evaluate the effect of a deficiency in dietary crude protein intake on postpartum reproductive performance of first-calf beef cows. Forty primigravid Hereford heifers were randomly assigned to two groups that were fed isocaloric (100% NRC requirements) diets containing .96 (adequate) or .32 (deficient) kg crude protein per head daily. Animals were individually fed from 150 d prepartum to 40 d postpartum and then were group-fed until the end of the experiment at 110 d postpartum. Total nutrient intake was increased by 33% at time of parturition to meet lactation requirements. Animals were artificially inseminated between 45 and 110 d postpartum when they showed estrus. Eighty-nine percent of those fed adequate protein showed estrus, whereas only 63% of protein-restricted heifers exhibited estrus (P less than .05). First-service conception (P less than .05; 71 vs 25%) and overall pregnancy rates (P less than .05; 74 vs 32%) were lower in protein-restricted heifers. Compared with those fed adequately, protein-restricted heifers had a tendency for longer intervals to first estrus (P less than .08; 75 vs 86 d), to first service (P less than .09; 76 vs 87 d) and to conception (P less than .09; 81 vs 92 d). Regardless of diet, weight at first estrus, weight at first service and average daily gain to 40 d postpartum were negatively correlated (P less than .01) with length of intervals from parturition to first estrus and first service.(ABSTRACT TRUNCATED AT 250 WORDS)

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