When preparing isolated rabbit retinas we found in some animals fundi which were not uniformly dark but had abnormal areas of red coloration. The in situ electroretinograms (ERG) of 82 rabbits recorded after 1 h of dark adaptation were checked for abnormalities indicative of a degenerative disorder. The ERGs of eight rabbits with small dark adapted b-waves (< or = 250 microV) were re-recorded and their b-waves found to decline with time. The greatest reduction, in three rabbits, was > or = 150 microV over 2.5 years. After 1 year, however, the light adapted b-waves were similar to those of rabbits with normal dark adapted b-waves. The majority of the progeny of these rabbits also had small b-waves, which became still smaller in 2 years. Ultrastructural studies of two rabbit retinas of the first generation showed pathological changes of the pigment epithelium (Wrigstad, Hanitzsch & Nilsson, Ultrastructural and electrophysiological studies of the retina and the retinal pigment epithelium in rabbits with low b-wave amplitudes, in preparation). Evidently there is an inheritable defect in the pigment epithelium which first impairs the rod pathway.

An increase in the amplitude of horizontal cell (HC) potentials, a decrease in the extracellular potassium around the photoreceptors and an increase in the PIII component of the ERG that occur with increasing light stimulus intensity are shown to be concomitant occurrences, which could, therefore, be causally related. With high intensity light stimuli the PIII component is prominent and the HC potentials exhibit an afterpotential. Within the range of intensities tested the peak times of the HC afterpotentials coincide with the peak times of PIII. This indicates the likelihood that the HC potential contributes to the PIII, although these results do not allow a quantitative assessment of the contribution of HC potentials to the PIII-component.

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