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The photocatalytic degradation of the chemical dye AZO and dye effluents in different time duration has been investigated using biologically synthesized silver nanoparticles. Dye industry effluents and AZO dye undergo degradation to form harmless intermediate and colourless products following irradiation by UV and solar light in the presence of green synthesized silver nanoparticles. The degree of degradation was tested under the experimental conditions such as PH, temperature, and absorbance of the dye in UV and solar light was measured. The degradation was higher in the UV light source than in the solar light source. Green synthesized silver nanoparticles in the UV light source were found to expedite the dye degradation process.

The present study deals with the nanoparticles synthesis from Impatiens balsamina L. plant flower extract. The concentration of reducing agent (flower extract) and different temperature is involved in the reaction. Sixteen different silver nanoparticles were synthesized with using different ratios of the reaction mixture and different temperature. The different silver nanoparticles are different color based on the temperature and concentration of reaction mixture. The synthesized silver nanoparticles are characterized by UV/Vis Spectrophotometer. The microorganisms Bacillus subtilis are highly inhibited by (90:10/60oC; 99:1/70oC; 92:2/70oC; and 90:10/70oC) silver nanoparticles. Pseudomonas aeruginosa are highly inhibited by 98:2/50oC and 90:10/70oC based silver nanoparticles. The bacterial species Staphylococcus aureus are highly inhibited by 98:2/50oC and 98:2/70oC. The E. coli was inhibited by 99:1/70oC; 98:2/70oC and 90:10/70oC based silver nanoparticles. The temperature and concentration of reducing agents can play a significant role in controlling the growth of nanoparticles. This study is one of the evidence on temperature and concentration of reducing agents to control the nanoparticles growth and their microbial inhibitory efficacy

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