The current study focuses on microwave‐assisted zinc oxide quantum dots synthesis (ZnO‐QDs) from zinc oxide bionanocomposite (ZnO‐BC) preparation. The novelty lies in the preparation of ZnO‐QDs, since the natural elements present in ZnO‐BC itself acted as a surface penetration enhancer without using any chemical agent. Under ultraviolet (UV) light ZnO‐QDs emitted a blue glow, confirming the fluorescence property. Using Box–Behnken design, the experimental factors of ZnO‐QDs were optimized, yielding a positive response of 350 nm absorbance and these results also matched with the UV‐visible spectroscopy characterization studies of ZnO‐QDs. Using Escherichia coli, the antibacterial activity of ZnO‐BC in comparison to ZnO‐QDs was determined using the well diffusion method and an inhibition zone ranging from 11 to 23 mm and in the broth assay the OD values were reduced by almost seven and 10 times for ZnO‐BC and ZnO‐QDs, respectively, when compared to the control (untreated). The antibacterial activity demonstrated that our newly prepared BC and its QDs have superior activity when compared to the standard antibiotics such as ampicillin. This type of nanomaterial can be used as a new bioactive natural material with light‐assisted activity for antibacterial coatings in the manufacture of personal protective equipment.
A green strategy and cost-effective approach was adapted to prepare Zinc oxide quantum dots (ZnO-QDs) for biomedical applications. The prepared ZnO-QDs may hold great promise as sensing scanners for diagnostics and therapy, as demonstrated in our current study. Zinc Sulphate, Azadirachta indica, and Catharanthus roseus leaves extract were used to synthesis a novel natural Zinc oxide bionanocomposite (ZnO-BC) and used as a precursor to prepare ZnO-QDs by microwave-assisted technique. The ZnO-BC was characterized by SEM-EDX, FTIR, XRD, Zeta potential and particle size analysis. The optical properties of QDs were investigated using UV and PL spectrophotometers. Experimental factors like the concentrations of ZnO-NPs, C. roseus and A. verawere evaluated using Box-Behnken design (BBD). MTT and haemolysis assay was performed using ZnO-BC and ZnO-QDs. Maximum absorbance observed at optimized values of 0.5% ZnO-NPs, 1g A.vera gel and 0.5ml C.roseus leaf extract of ZnO-QDs against BBD. There was decreased viability rate, ranging from 60-15% for 0.5mg/ml ZnO-BC and 45-5% for 5 mg/ml ZnO-QDs which revealed a tenfold decrease in cell viability with less concentration scale for 5mg/ml of ZnO-QDs when compared with that of 0.5 mg/ml ZnO-BC. Also, hemolysis test shows that the hemolysis ratio was below 0.5%, indicating non-haemolysis of ZnO-QDs. cellular morphology by results was supported by phase-contrast microscopy images. A good biocompatibility and high anticancer activity was noticed for ZnO-QDs when compared to ZnO-BC and provide versatile applications in the eld of Nano biomedicine.
The recent study explains about the removal of Pb (II) ions from aqueous solution using activated carbon prepared from Garlic waste. Garlic peels have been used for the production of Carbon by treating with conc.H2SO4 for metal ions removal. Fourier Transform Infrared Spectroscopy and Boehm titration have been used for various physicochemical characterization of the outcome of activated carbon which proclaimed the presence of oxygen containing surface functional groups like phenolic, lactonic and carboxylic in the carbons. In a batch adsorption process the effect of pH and initial metal ion concentration was calculated. The optimum pH for lead adsorption is found to be equal to 6.The resultant activated carbon showed maximum adsorption capacity of Pb (II) was 210 mg g-1. The waste material which is used in this work is cost effective and easily available for the production of activated carbon. Hence the removal of Pb (II) from water using the carbons prepared from Garlic peels can act as possible low cost adsorbents for the removal of Pb (II) from water.
A green strategy and cost-effective approach was adapted to prepare Zinc oxide quantum dots (ZnO-QDs) for biomedical applications. The prepared ZnO-QDs may hold great promise as sensing scanners for diagnostics and therapy, as demonstrated in our current study. Zinc Sulphate, Azadirachta indica, and Catharanthus roseus leaves extract were used to synthesis a novel natural Zinc oxide bionanocomposite (ZnO-BC) and used as a precursor to prepare ZnO-QDs by microwave-assisted technique. The ZnO-BC was characterized by SEM-EDX, FTIR, XRD, Zeta potential and particle size analysis. The optical properties of QDs were investigated using UV and PL spectrophotometers. Experimental factors like the concentrations of ZnO-NPs, C. roseus and A. verawere evaluated using Box-Behnken design (BBD). MTT and haemolysis assay was performed using ZnO-BC and ZnO-QDs. Maximum absorbance observed at optimized values of 0.5% ZnO-NPs, 1g A.vera gel and 0.5ml C.roseus leaf extract of ZnO-QDs against BBD. There was decreased viability rate, ranging from 60-15% for 0.5mg/ml ZnO-BC and 45-5% for 5 mg/ml ZnO-QDs which revealed a tenfold decrease in cell viability with less concentration scale for 5mg/ml of ZnO-QDs when compared with that of 0.5 mg/ml ZnO-BC. Also, hemolysis test shows that the hemolysis ratio was below 0.5%, indicating non-haemolysis of ZnO-QDs. cellular morphology by results was supported by phase-contrast microscopy images. A good biocompatibility and high anticancer activity was noticed for ZnO-QDs when compared to ZnO-BC and provide versatile applications in the field of Nano biomedicine.
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