SummaryThe relative efficiencies of organic, polar solvents and of solvent‐water pairs for use in the extraction of gossypol and related compounds from cottonseed flakes were determined in a specially devised glass laboratory extractor.Of the solvents tested a butanone‐water pair containing 10% of water by volume was the most effective, and chlorine‐substituted hydrocarbons were the least effective. Under equilibrium conditions maximum extraction of gossypol was obtained with a butanone solvent containing 2.5% of water by weight. The rate of extraction of gossypol from cottonseed meal with butanone‐water pairs increased with increase in the amount of water in the system and with increase in temperature of the extraction system. The greater amounts of water in the extraction system resulted in swelling and packing of the flakes and in a decrease in extraction efficiency. Flakes extracted at 26°C. contained 0.08% free gossypol and those extracted at 71°, 0.054%. This decrease may be due, in part, to the reaction of gossypol with the protein to form bound gossypol.

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SummaryTreatment of freshly prepared crude cottonseed oils withp‐aminobenzoic acid and subsequent removal of the di‐p‐carboxyanilinogossypol formed makes it possible to store the oils at a relatively high temperature (37–38°C.) and for an extended period of time (30 days) without incurring any adverse changes in the refining and bleaching properties of the oils. In addition, a considerable decrease in the refining loss of the crude oil is obtained, and the stability of the bleached oil is not affected by the treatment.Spectrophotometric studies made during all phases of the chemical treatment and during the refining and bleaching procedures show that thep‐aminobenzoic acid removes almost completely the gossypol‐like pigments which are present in the crude oils and yields oils having the characteristic carotenoid spectrum.

SummaryWith different types of cottonseed products and cottonseed products to which gossypol is added, there is a loss or disappearance of gossypol upon storage, as determined analytically. The rate of disappearance of gossypol varied for the different type cottonseed and was increased at higher temperatures and over the longer periods of storage.When gossypol is incorporated into different types of animal diet materials, there is a loss or inactivation of some portion of the added gossypol as determined analytically immediately after preparation of the gossypol‐diet mixtures, and a further additional loss or inactivation upon storage. The factors which may contribute to this loss or inactivation of gossypol are: a) the components of the diet materials; b) the temperature and length of time of storage; c) the concentration of gossypol added and the final percentage of gossypol in the mixture.