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High temperature causes alteration in physiological and yield parameters of mulberry plant. The present study was conducted to examine the tolerance/ susceptibility of five popularly cultivated mulberry varieties viz., V 1 , G 2 , G 4 , MR 2 and S 36 exposed to high temperature stress. The varieties were maintained in Open Top Chambers (OTCs) at 40 °C for two weeks. It is found that the variety V 1 was tolerant to high temperature stress followed by MR 2 and S 36 . After 14 days of high temperature stress, significant differences were observed among the varieties for leaf yield per plant under control and stress conditions compared to control and 7 th day after stress. The variety G 2 and G 4 recorded highest reduction in Total Dry Matter Accumulation (TDMA) (55.60% and 55.96%) at 14 th day after stress. Whereas, the lowest reduction percent of TDMA was recorded in V 1 (23.06%) followed by MR 2 (30.80%) and S 36 (36.76%) compared over its control values at 14 th day after stress.