The objective of this study was to improve the dissolution and solubility of dexibuprofen (DEX) using hydroxypropyl beta cyclodextrin (HPβCD) inclusion complexes and also to evaluate the effect of presence of hydrophilic polymers on solubilization efficiency of HPβCD. Three different methods (physical trituration, kneading and solvent evaporation) were used to prepare binary inclusion complexes at various drug-to-cyclodextrin weight ratios. An increase in solubility and drug release was observed with the kneading (KN) method at a DEX/HPβCD (1:4) weight ratio. The addition of hydrophilic polymers poloxamer-188 (PXM-188) and poloxamer-407 (PXM-407) at 2.5, 5.0, 10.0 and 20% w/w enhanced the complexation efficiency and solubility of DEX/HPβCD significantly. Fourier-transform infrared (FTIR) analysis revealed that DEX was successfully incorporated into the cyclodextrin cavity. Differential scanning calorimetry (DSC) and X-ray diffractometry (XRD) revealed less crystallinity of the drug and its entrapment in the cyclodextrin molecular cage. The addition of PXM-188 or PXM-407 reduced the strength of the DEX endothermic peak. With the addition of hydrophilic polymers, sharp and intense peaks of DEX disappeared. Finally, it was concluded that PXM-188 at a weight ratio of 10.0% w/w was the best candidate for improving solubility, stability and release rate of DEX.

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Orodispersible sublingual films (OSFs) composed of hydrophilic polymers were loaded with poloxamer-188 and d-α-tocopheryl polyethylene glycol succinate (TPGS-1000) mixed micelles to improve the oral bioavailability of a poorly soluble drug, ebastine (EBT). Mixed micelles formed by thin-film hydration method were incorporated into orodispersible sublingual film, consisting of HPMC and glycerol, using solvent casting technique. The mixed micelles and films were thoroughly evaluated for physicochemical characterization (size, polydispersity index, zeta potential, entrapment efficiency, thickness, weight, surface pH studies, disintegration time, swelling indices, mechanical properties, FTIR, PXRD, DSC, SEM, AFM, in vitro drug release, in vivo bioavailability, and toxicological studies). The results showed that the average particle size of mixed micelles was 73 nm. The mean zeta potential and PDI of the optimal mixed micelles formulation were −26 mV and 0.16, respectively. Furthermore, the maximum entrapment efficiency 82% was attained. The film’s disintegration time was in the range of 28 to 102 s in aqueous media. The integrity of micelles was not affected upon incorporation in films. Importantly, the micelles-loaded films revealed rapid absorption, high permeability, and increased bioavailability of EBT as compared to the pure drug. The existence of ebastine loaded mixed micelles in the films enhanced the bioavailability about 2.18 folds as compared to pure drug. Further, the results evidently established in-vitro and in-vivo performance of bioavailability enhancement, biocompatibility, and good safety profile of micelles-loaded orodispersible EBT films. Finally, it was concluded that film loaded with poloxamer-188/TPGS-1000 mixed micelles could be an effective carrier system for enhancing the bioavailability of ebastine.

The augmented block design (ABD) is commonly used in breeding programs to evaluate the performance of large number of genotypes using non-replicated new test treatments and replicated check treatments. The purpose of this design is to determine which test treatment performs better than check treatments. The objective of this study is to identifying better genotype of Mash been than check treatment under rain feed condition of Pakistan. For this purpose, the data of 125 mash been genotypes included 5 check entries were analyzed. The experiment was conducted in augmented block design using 6 blocks in rain feed condition of Pakistan and data of different attribute were recorded. The analysis showed that the treatment effects (adjusted) are significant for all attributes. However, for all attribute other than the number of seeds/pod, the adjusted block effects are non-significant, indicating that the evaluated blocks are homogeneous. Only three principal components were considered, first component explained 33.85% of variation and 21.85%, 17.51% explained by PC2 and PC3 respectively for the first season whereas in the second season 33.62%, 23.43%, and 16. 86% variation described with respect to the first, second, and third principal components. First PC designated as a component of productivity, second as component of pod appearance while third designed as a component of physical appearances. All attributes showed significant and positive association with other attributes, only significantly negative association was among number of pods/plant and100-seeds weight.

The study's objectives was to determine the frequency of various clinical manifestations of congenital adrenal hyperplasia in children. Materials & Methods: The research methodology is descriptive and cross sectional. The event was held at the Department of Pediatric Medicine at DHQ Hospital in Faisalabad. The study will last from September 29th to March 28th, 2019. A total of 90 children with congenital adrenal hyperplasia of age 0-10 years of either gender were included. After taking informed written consent from all children’s parents, different clinical presentations (ambiguous genitalia, simple virilising and salt wasting) were noted in each patient. Results: Mean age of children was 4.84 ± 1.78 years. Out of the 90 patients, 62 (68.89%) were male and 28 (31.11%) were females with male to female ratio of 2.2:1. Mean duration of symptoms was 5.79 ± 2.59 days. In this study, I have found the ambiguous genitalia in 27 (30.0%), simple virilising in 17 (18.88%) and salt wasting in 59 (65.56%) children with congenital adrenal hyperplasia. Conclusion: This study has shown that the salt wasting is the most common clinical presentations of congenital adrenal hyperplasia in children followed by ambiguous genitalia and simple virilising. Keywords: Congenital adrenal hyperplasia, ambiguous genitalia, salt wasting.