Aim : The study was conducted to reveal the efficacy of Bone marrow derived mesenchymal stem cells (BM-MSCs) based therapy in healing of chronic non-healing and ulcerative wound in bovine species. Materials and Methods: One 2 years old Jersey heifer affected with chronic ulcerative wound involving full thickness skin and under lying muscle at dorsal side of lumbar region since four months at the time of presentation. Bone marrow was collected from tibia, cultured and grown and after achievement of optimum confluence it was applied at the site. Different parameters of clinical, physiological, haematological, biochemical, histochemical, histological, tensile strength and photographic evaluations were done during the study period. Results:The estimated values of above mentioned parameters on zero day and after healing (18 days) showed significant difference (P<0.05) in relation to collagen content, tensile strength and physical characteristics of wound like extent of wound, size of wound, type of exudates and photography. But clinical, haematological and biochemical data showed no significant difference. Conclusion: The BM-MSCs were the main pioneers to bring the chronic ulcerative wound towards healing. The procedure is simple, safe and effective in bringing out healing without showing any adverse effect on host. Key words: BM-MSCs, bovine, chronic ulcerative wound healing. Hence one clinical trial was made with Introduction autologous bone marrow derived stem cell (BMChronic non-healing or slow healing ulcers MSCs) therapy and to know the progress and duration represent a major health burden and continue to pose a of healing with this application. challenge not only to the patient owners but to the Materials and Methodstreating physician and medical procedures as a whole. The healing of wound occurs by primary intention andThe owner was fully informed and written secondary intention [1,2,3]. The therapeutic application consent was obtained about the clinical trial, risk and of autologous BM derived MSCs (Bone marrow benefits of the proposed cell based therapy. It was also derived mesenchymal stem cells) have revolutionized approved by the Institutional Animal Ethics the field of regenerative medicine. The BM-MSCs Committee of the institution since it was a clinical case were been used for the treatment of chronic wound in and related to Agricultural Production Research. diabetic patients [4], for non-healing ulcers of lower Measurement of the wound dimension was taken extremities in human being [5], cutaneous radiation along with swab from wound bed for bacteriological syndrome in minipig model [6] and in dogs [7] proved culture and sensitive test (Fig.1). Biopsy was done successful in their findings. A clinical case of 2 years from wound bed for histopathological and old Jersey heifer was presented with one large size histochemical study. Wound was washed, cleaned and chronic non-healing wound at lumbar region since 4 dressed with fly repellent spray. Under peroneal nerve months. It was treated with dif...

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A 5-year-old male dog was presented with the complaint of a chronic nonhealing wound on both hind legs. The wound occurred during a serious train accident that caused loss of both hind limbs below the hock joint. Since then, the wound did not respond to any standard treatment for up to 4 months. Again it was dressed for 15 days, but it did not show any improvement. With the consent of the owner, cell-based therapy was conducted by collecting bone marrow from the proximal anteromedial aspect of the tibia. After the application of bone marrow derived mesenchymal stem cells (BM-MSCs) the time to complete healing was 24 days. Due to pressure at the bony cut stump, the dog was noted to have pain while walking, and walked on his forelegs. An artificial limb was subsequently applied. The dog managed to walk normally after several days of training.