A 11 ecosystems and human societies depend on a healthy and tlroductive natural environment that contains diverse plant and animal species. The earth's biota is comtlosed of an estimated 1 0 million species of plants, animals, and microbes (Pimm et al. 1995). In the United States, there are an estimated 750.000 stlecies. of which small organisms, such as arthropods and microbes, make up 95%.' Although approximately 60% of the world's food supply comes from rice, wheat, and corn (Wilson 1988), as many as 20,000 other plant species have been used by humans as food. Some vlants and akimals provide human; with essential medicines and other diverse, useful ~r o d u c t s. For instance. some plants i n d microbes help to d&rade chemical pollutants and organic wastes and recycle nutrients throughout the ecosystem. The rapidiy growing world population and increased human activity threaten many of these species. The current extinction rate of species ranges from approximately 1000 to 10,000 times higher than natural extinction rates (Kellert and Wilson

This paper focuses on YOLO-LITE, a real-time object detection model developed to run on portable devices such as a laptop or cellphone lacking a Graphics Processing Unit (GPU). The model was first trained on the PASCAL VOC dataset then on the COCO dataset, achieving a mAP of 33.81% and 12.26% respectively. YOLO-LITE runs at about 21 FPS on a non-GPU computer and 10 FPS after implemented onto a website with only 7 layers and 482 million FLOPS. This speed is 3.8× faster than the fastest state of art model, SSD MobilenetvI. Based on the original object detection algorithm YOLOV2, YOLO-LITE was designed to create a smaller, faster, and more efficient model increasing the accessibility of real-time object detection to a variety of devices.

This study was designed to determine whether nutritional folate depletion exerts hepatic oxidative stress in relation to elevated plasma homocysteine. To mimic various extents of folate depletion status in vivo, male Wistar rats were fed an amino acid-defined diet containing either 8 (control), 2, 0.5, or 0 mg folic acid/kg diet. After a 4-wk feeding period, the plasma and hepatic folate concentrations of the rats decreased significantly with each decrement of dietary folate. Folate depletion did not significantly affect two major liver antioxidants: reduced glutathione and alpha-tocopherol. Conversely, folate depletion decreased Cu-Zn superoxide dismutase and glutathione peroxidase activities, but had no effect on catalase activity in liver homogenates. Lipid peroxidation products, as measured by thiobarbituric acid-reactive substances, were significantly higher in livers of folate-depleted rats than in those of the controls. This occurrence of hepatic oxidative stress in folate-depleted rats was confirmed by demonstrating an increased susceptibility of livers of folate-depleted rats to lipid peroxidation induced by additional H2O2 or Fe(2+) treatments compared with the controls. Decreasing dietary folate intake resulted in graded increases in plasma homocysteine concentrations of folate-depleted rats. Elevated plasma homocysteine and decreased plasma and hepatic folate concentrations in folate-depleted rats were all strongly and significantly correlated with increased liver lipid peroxidation (/r/ > or = 0.58, P < 0.0003). These data demonstrate that folate depletion and elevated plasma homocysteine promote oxidative stress in rat livers.

Objective Nucleated red blood cells (NRBCs) have been identified in maternal circulation and potentially provide a resource for the monitoring and diagnosis of maternal, fetal, and neonatal health and disease. Past strategies used to isolate and enrich for NRBCs are limited to complex approaches that result in low recovery and less than optimal cell purity. Here we report the development of a high-throughput and highly efficient microfluidic device for isolating rare NRBCs from maternal blood. Material and Methods NRBCs were isolated from the peripheral blood of 58 pregnant women using a microfluidic process that consists of a microfluidic chip for size-based cell separation and a magnetic device for hemoglobin-based cell isolation. Results The microfluidic–magnetic combination removes nontarget red blood cells and white blood cells at a very high efficiency (∼99.99%). The device successfully identified NRBCs from the peripheral blood of 58/58 pretermination samples with a mean of 37.44 NRBC/mL (range 0.37–274.36 NRBC/mL). These results were compared with those from previous studies. Conclusion The microfluidic device results in an approximate 10- to 20-fold enrichment of NRBCs over methods described previously. The reliability of isolation and the purity of the NRBC product have the potential to enable the subsequent application of molecular diagnostic assays.

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