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The boron (B) availability and uptake were studied in relation to different phosphorus rates applied into soils in a three-year field experiment (2015–2017). The experiment was carried out at the experimental station at Humpolec (Bohemian-Moravian Highlands, Czech Republic). Three rates of phosphorus (20-40-80 kg P/ha) were applied as triple superphosphate. The crop rotation was spring barley-winter oilseed rape-winter wheat. No systematic fertilization with B was used and the response of natural boron soil content to the different phosphorus supply was studied. The crop yields, B content in plants, B-uptake, and content of B (extracted by Mehlich 3 and NH₄ acetate methods) were determined. Spring barley and winter wheat B uptake was about one order of magnitude lower in comparison with oilseed rape. Significant differences in B content in soils, in crop tissues and B-uptake, were found mainly under higher phosphorus doses (40 and 80 kg P/ha). NH₄ acetate method showed better correlations between P and B contents in soils than Mehlich 3 method from the second experimental year. The P-fertilization may affect negatively the B-uptake by plants, particularly if the highly nutrient demanding crop is grown.

The boron (B) availability in soils, B uptake and possible B interactions with phosphorus (P) were studied in a pot experiment with increasing P rates. Three soil types of different characteristics were used for the experiment that lasted two years. The two soil tests were used in the experiment – Mehlich 3 and NH₄-acetate. Significant positive correlations were found between barley dry matter yield, B content in plants and B uptake under increasing P treatments (P ≤ 0.001). The significant relationship for B content (P ≤ 0.001) was obtained between the used soil tests for all tested soils. A decrease of soil B content in soils under increasing P doses was determined. NH₄-acetate soil test showed a significant relationship between B and P contents (P ≤ 0.001) within the studied soils in comparison with the Mehlich 3 method. Considering individual soils, the NH₄-acetate test showed more often higher significance in comparison with the Mehlich 3 test. The interactions between P and B in soils should be taken in consideration when deciding about the phosphorus fertilization.