The scaffolds and their interaction with mesenchymal stem cells are objects of study in bioengineering and tissue repair. Mechanisms such as surface adhesion, proliferation, viability, and cytotoxicity are essential for the development of therapies. The present study analyzed the influence of platelet-rich fibrin (PRF) in viability, cytotoxicity, and proliferation of stem cells from human exfoliated deciduous teeth (SHED) exposed to bovine biomaterial surfaces. The studied groups were divided and analyzed as follows: (S) only SHED as control Group; (SB) SHED + biomaterial; (SBP) SHED + biomaterial + PRF. Analyses of cells seeded in 24-well plates were performed after 24, 48 and 72 hours. Individual groups were subjected to viability, cytotoxicity and cell proliferation tests using neutral red, MTT and crystal violet, respectively; and in the 72-hour group, scanning electron microscopy (SEM) was performed to record cell ultra-morphology. Data were submitted to statistical analysis by two-factor ANOVA with a significance level of 5%. The results demonstrated a better performance in the viability/cytotoxicity and proliferation of stem cells for the group (SBP) in comparison to the group (SB) and the group (S). The applied statistical tests showed that the biomaterial factor, time, and interaction between them gave rise to results with statistical significance. SHED submitted to bovine biomaterial were more viable, proliferative and with lower toxicity when associated with PRF. PRF seemed to activate the metabolism of stem cells in culture, indicating that such an association can bring an effective benefit in clinical outcome.

A bichectomia é um procedimento que se constitui na remoção da porção anterior do corpo adiposo de Bichat (paCAB). O extrato celular obtido após o processamento por meio de digestão enzimática desse tecido é chamado de fração vascular estromal (FVE). A natureza das células que compõem a FVE qualifica esse produto para uma ampla gama de aplicações clínicas, especialmente em procedimentos de estímulo à renovação e à reparação tecidual, incluindo-se os fins estéticos de rejuvenescimento em face. Entretanto, o aproveitamento desse material biológico vivo está diretamente relacionado ao seu adequado manejo e transporte, desde sua coleta, processamento e envio para aplicação clínica. Este trabalho teve como objetivo caracterizar a FVE obtida a partir da paCAB, e também verificar se o plasma pobre em plaqueta (PPP) autólogo é eficiente na manutenção da viabilidade celular, podendo ser uma opção de meio para transporte até sua aplicação clínica mediata. Três pacientes com indicação da realização de bichectomia participaram da pesquisa. Antes da remoção da paCAB foi realizada a coleta sanguínea venosa para a obtenção do PPP. A paCAB bilateral coletada foi enviada para o Centro de Processamento Celular Curityba Biotech e submetida ao protocolo para desagregar as células da matriz extracelular. Ao final, a FVE foi fracionada em alíquotas que foram acondicionadas em seringas e em placas de cultivo, e impostas a ensaios para se avaliar a viabilidade celular nos tempos 0, 24 e 48 h. A viabilidade celular e a caracterização das células presentes na FVE foram avaliadas por microscopia de luz e imunofenotipagem por citometria de fluxo. Uma amostra foi mantida em garrafa de cultivo até atingir 7x106 células. Após, foram  impostas  a comprovação da presença de células-tronco mesenquimais (CTM) capazes de se manter em cultivo padrão. A amostra analisada por imunofenotipagem confirmou a existência das seguintes células: células-tronco mesenquimais e hematopoiéticas, células endoteliais e células T.

Eyesight can be considered a major human interaction promoter in motor, perceptual and mental activities and its loss can cause social environment changes. Objective: To evaluate and determine Simplified Oral Hygiene Index (OHI-S) in visually impaired individuals, as well as to provide information to promote oral health, to verify the effectiveness of educational activities for index change and to promote the social inclusion of visually impaired people. Material and methods: Study population was constituted by 28 visually impaired people of both genders, aged from 14 to 75 years old, residents and students at the Parana Institute for the Blind (IPC), Curitiba (PR, Brazil). The study was conducted in three stages. At first, the participants individually answered a questionnaire, performed tooth brushing and then the disclosure of oral biofilm was made, as well as the evaluation of Simplified Oral Hygiene Index, oral hygiene instruction and supervised toothbrushing. The second step was performed after seven days and the third after thirty days to reassess the Simplified Oral Hygiene Index to check whether there would a change in their values. Participants were divided into two groups according to Simplified Oral Hygiene Index: those who had only bacterial plaque and those who had plaque and calculus. Results: At the first day, patients with plaque and calculus had initial OHI-S mean of 2.3 (regular), 2.1 (regular) after seven days, and 2.4 (regular) after one month. Patients with only plaque at the first day had an average OHI-S of 0.71 (regular), 0.74 (regular) after seven days, and 0.78 (regular) after one month. Conclusion: Within this context, it is possible to understand that it is necessary to implement frequent supervised toothbrushing, oral instruction and motivation activities to promote wellness and health for these patients.