The bacterial isolates were obtained from Al-Kindi Hospital were diagnosed by the Vitek-2 system and re confirm by 16srRNA gene as S. aurous, the results were shown 20 isolates (66.7%) out of 30 isolates were positive to protease production. All bacterial isolates (100%) were sensitive to Gentamicin and Levofloxacin. but resistant (100%) to aztreonam. The best temperature for enzyme production from bacteria was 37 °C, and the best pH for enzyme production was 7. Partial purification of the bacterial enzyme (protease) was carried out using short steps included ammonium sulfate 65% saturation, ion exchange using DEAE- cellulose column and then applied on gel filtration chromatography using Sephadex G-200 column. The enzymatic activity was determined for each purification step. The specific fold and yield of the purified enzyme were 5.91 and 38.3 % respectively. The molecular weight of the purified enzyme was 37 kDa , it was determined by SDS-PAGE. After being exposed to high concentrations of the protease enzyme (800-1000 µg/ml), the proliferation of a breast cancer cell line (MCF-7) was seen to be suppressed, however the inhibitory effect gradually diminished as the concentration of the enzyme decreased. 200–400 µg/ml is the recommended concentration.

Urinary Tract Infections (UTIs) are one of the most common bacterial infections, with a frequency of 50%-60% in mature females [1] Urinary tract infections could be uncomplicated or complicated. Uncomplicated UTIs affects individuals with no neurological or structural deformities in urinary tract. Uncomplicated UTIs could be separated into infection of lower UTIs (cystitis) and upper UTIs (Pyelonephritis) [2]. Underlying factor for cystitis are gender, former UTI infection, genetic susceptibility, vaginal infection, sexual activeness, overweight and diabetes [3], whereas uncomplicated UTIs arises from presence of factors debilitates host immune defenses including renal transplantation, renal failure, urine retention due to neurological disorders, immunosuppression, presence of foreign bodies with urinary tract like indwelling catheters [4]. In the United states, the indwelling catheters results in 70-80% of complicated UTIs [5] with a total of one million cases per year [6]. Etiology of UTIs includes Gram-negative , gram-positive and fungi. Uropathogenic Escherichia Coli (UPEC) is the most common bacterial of all types of UTIs. In the cases of Uncomplicated UTIs, UPEC is followed in frequency by K. pneumoniae, S. saprophyticus, Enterococcus faecalis, Group B Streptococcus (GBS), Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida spp. [7], whereas in complicated UTIs, UPEC is followed in frequency by Enterococcus spp., K. pneumoniae, Candida spp., S. aureus, P. mirabilis, P. aeruginosa and GBS [8]. The emergence and exacerbation of the bacterial drug resistance problem, beside other problems of antibiotic therapy as the adverse effects of hepatoxicity, nephrotoxicity , ototoxicity, mutagenicity and carcinogenicity; immunosuppression; eradication of benefi cial gut and mucosal surfaces fl ora and allergic reactions [9], made most of antibiotics worthless in treatment of many cases of UTIs and directed global attention towards fi nding new therapeutic alternatives. Herbal medicine is the most important alternative therapy for classical antibiotics. Scientifi c research on herbal medicine confi rmed the therapeutic activity of vast majority of medicinal plants known in traditional medicine of different areas worldwide for their activity in treatment microbial infections and different human diseases [10,11].

SAR-COV2 is still a pressing issue, 219M people were infected and more than 4.5M lost their lives. The majority of antiviral and inflammatory therapies could only provide a supportive role in treating a limited number of COVID cases. This review investigates the available vaccines in terms of their safety and efficiency in fighting the virus. Seven vaccines are similar in their side effects to other influenza vaccines and their necessity to a booster dose. Although that several technologies have been used to manufacture the vaccine, mRNA vaccines clearly show a high protection rate touched 90% specially in severe and hospitalization cases prevention. Among all available vaccines, Pfizer vaccine is an exception as it granted the full approval to be used in people age 16 and under till five years.