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To study biochemical changes of coconut inflorescence sap on storage for one week in different varieties. Methods and Results:The experiment was conducted at College of Agriculture, Padannakkad and Nileswaram farm, RARS Pilicode under irrigated condition during 2014-2016. Four varieties were selected for evaluation. Palms with similar age and morphological characters were selected as experiment units. The varieties tried were Malayan Yellow Dwarf, Kerasree, West Coast Tall and Keraganga. Fully emerged unopened bunches were selected for tapping and samples were collected. The sap collected from each variety was stored at refrigerated condition for one week and biochemical properties viz., pH, electrolyte concentration, reducing sugars, non-reducing sugars, total sugars, alcohol, phenol and vitamin C were assessed on 1st (D1), 3rd (D2) and 7th (D3) days of storage. The results are revealed that without any differentiation between the varieties there was a significant reduction in the pH, total sugar, reducing sugars and non reducing sugar while there was an increase in alcohol, vitamin C and phenol content. Conclusions:The changes in CIS during storage for 1 week indicated that without any differentiation between varieties there was a decrease in pH, total sugar, reducing sugar and non-reducing sugar while there was an increase in alcohol and vitamin C content. Significance and Impact of the study: To know the changes in biochemical properties of coconut inflorescence sap of different varieties on storage whereas there is no variation among the varieties with respect to biochemical properties of coconut inflorescence sap on storage.