This study was conducted to evaluate seed morphogenesis and effect of pretreatments on seed germination of Persian shallot or mooseer (Allium hirtifolium Boiss.). In a first experiment, seed developmental stages of Persian shallot (Mooseer) were assessed. Results showed that number of ovules varied among locules and flowers. In most cases 3-4 ovules occurred per locule and developed into 1-2 seeds and therefore, a ripe capsule usually contained 3-6 seeds. The seeds were black in color and ranged from 3.73-3.98 mm in length and 2.75-3.16 mm in width, and the average weight of 1,000 seeds varied between 7.80-8.20 grams. In a second experiment four factors, scarification, stratification, potassium nitrate, and GA(3), were evaluated for seed germination. Results showed that seed germination was not observed without sandpaper scarification or stratification treatment. It could be concluded that the best treatment for overcoming mooseer seed dormancy and getting the longest seedling length was scarification with sandpaper, GA(3) (500 mg L-1 for 12 hours) application, and moist stratification. The results obtained in the present study revealed that mooseer seed has mechanical dormancy and only cold stratification is needed for endosperm weakening
We evaluated the genetic diversity of Iranian Mooseer (Allium stipitatum Regel. syn. A. hirtifolium Boiss.) using morphological traits and AFLP markers and determined the phylogenetic relationships and the position of Iranian Mooseer among domesticated and wild edible alliums using internal transcribed spacer (ITS) sequence data. Four EcoRI and MseI AFLP primer combinations rendered produced 376 markers, 204 being polymorphic (53.28% polymorphism). AFLP data separated clearly the Kazeroun accession, originating from the warmer Southern region of Iran, from the other 20 accessions. According to the morphological evaluation data, Khansar, Kouhrang and Daran accessions showed the highest means for bulb weight. Cluster analysis based on morphological traits divided the Mooseer germplasms into three main groups. The ITS results indicated that Iranian Mooseer clustered with other A. stipitatum accessions, but with small differences. They were phylogenetically close to Iranian wild edible alliums and clearly differentiated from other domesticated alliums. Separation of three Iranian Mooseers from A. stipitatum samples originated from other countries in two distinct groups according to their ITS sequence shows that probably these two groups have divergent evolutionary paths.
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