Background: Delayed onset muscle soreness (DOMS) is the sensation of stiffness and pain in the muscles that occurs 24-48 hours after unaccustomed or strenuous exercise. Objectives: The aim of this study was to examine and compare the effect of vibration training (VT) on Delayed onset muscle soreness. Materials and Methods: Twenty healthy non-athletic volunteers (Female, age 20-30 years, body mass 23.27 ± 0.82 kg) were randomly assigned to VT (n = 10) and non-VT (n = 10) groups. A vibrator was used to apply 50-30 Hz of vibration on the left quadriceps and calf muscles in the VT group, while no vibration was applied for the non-VT group. Then, both groups ran downhill on a -5° treadmill at a speed of 5-7.5 km/hour (increasing speed). Twenty-four-hours before and after the vibration training, serum creatine kinase levels, pressure pain threshold and the rate of edema were measured. Results: Compared with the non VT group, the VT group showed a significant decrease in pressure pain threshold at 15 cm of patellar point (P = 0.042) and calf muscle (P = 0.041), there were no significant differences between groups in the pressure pain threshold at 5 cm (P = 0.509) and 10 cm (P = 0.152) points. There was no significant change in the edema rate at the Gastrocnemius muscle (P = 0.420) and midpoint of quadriceps muscle (P = 0.380) in VT and non-VT groups. Despite the increase in creatine kinase enzymes in the two groups, there was no statistically significant difference between the groups (P = 0.173). Conclusions:The results showed that using the vibration training can affect delayed onset muscle soreness (DOMS) reduction between non-VT and VT groups. Further studies with larger samples are needed to determine the vibration training positive and negative effects.