Water quality is one of the main characteristics of a river system and prediction of water quality is the key factor in water resource management. Different physical, biological and chemical parameters including heavy metals can be used to assess river water quality. Evaluation of the water quality in the rivers is quite difficult and requires more time and effor t because of the fact that many factors affect water quality. Traditional data processing methods are insufficient to solve this problem. Therefore, in this study, an adaptive neuro-fuzzy inference system (ANFIS) model was developed to predict the concentrations of cadmium (Cd) in the Filyos River, Turkey. For this purpose, water samples collected at 7 sampling locations in the river during December 2014-2015 were used to develop ANFIS model. The available data set was apportioned into two separate sections for training and testing the ANFIS model. Developed models aimed to use the least parameters to estimate Cd concentration. As a result, a relatively higher correlation (R 2 =0.91) was found between observed and modelled Cd concentrations. The results indicated that the ANFIS model gave reasonable estimates for the concentrations of Cd with a high degree accuracy and robustness. In conclusion, this paper suggests that ANFIS methodology produce very successful findings and has the ability to predict Cd concentration in water resources. The outcomes of this research provide more information, simulation, and prediction about heavy metal concentration in natural aquatic ecosystems. Therefore, ANFIS can be used in further researches on water quality monitoring.

The pharmacokinetic of enrofloxacin was investigated in brown trout (Salmo trutta) following oral administration of 10, 20, and 40 mg/kg doses at 11 ± 1.5 °C. Furthermore, MICs of enrofloxacin against Aeromonas hydrophila and A. sobria were determined. The plasma concentrations of enrofloxacin and ciprofloxacin were determined using HPLC–UV and analyzed by non-compartmental method. Following oral administration at dose of 10 mg/kg, total clearance (CL/F), area under the concentration–time curve (AUC0−∞) and peak plasma concentrations (Cmax) were 41.32 mL/h/kg, 242.02 h*μg/mL and 4.63 μg/mL, respectively. When compared to 10 mg/kg dose, the dose-normalized AUC0–∞ and Cmax were increased by 56.30% and 30.08%, respectively, while CL/F decreased by 38.4% at 40 mg/kg dose, suggesting the non-linearity. Ciprofloxacin was not detected in the all of plasma samples. The MIC values of enrofloxacin were ranged 0.0625–4 μg/mL for A. hydrophila and 0.0625–2 μg/mL for A. sobria. The oral administration of enrofloxacin at 10 (for 192 h) and 20 (for 240 h) mg/kg doses provided the AUC of enrofloxacin equal to 1.23 and 1.96-fold MICs, respectively, for A. hydrophila and A. sobria with the MIC90 values of 1 µg/mL. However, further researches are needed on the PK/PD study of enrofloxacin for the successful treatment of infections caused by A. hydrophila and A. sobria in brown trout.

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The present study aimed to obtain gynogenetic zebrafish. For this purpose, zebrafish spermatozoa exposed to UV irradiation to make it haploid gynogenetic fish and heat shock was applied to haploid zygote in order to obtain gynogenetic diploid fish.Temperature, which is an important factor of the production, is taken into consideration in this study. In this respect, this study compared the results of 41.4°C and 41°C heat-shock applications and found that 12nd-24th-48th-72nd hour survival rate was maximum at 41,4°C (P<0.05). When considered from hatching rate (72th-78th hour) view at 41.4°C was 17.3 ± 3% in gynogenetic diploid group and heat-shock application at 41°C survival rate was 14 ± 2% in gynogenetic diploid group and there is no survivor in haploid group, was observed (P<0.05).The result of the karyotype analysis in haploid gynogenetic embryos, ruptured chromosome fragments was identified. Also in karyotype analysis of diploid gynogenetic embryos, 2n=50 chromosomes was identified. On 3rd day after fertilization, total body length of the haploid gynogenetic fish was 39.6% shorter and body thickness was 33% thicker than the diploid gynogenetic group. Our gynogenetic fish producing method and the different heat-shock applications improved survival rate of gynogenetic fish.

The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1-1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. In the in vivo trials, fertilized eggs were treated with predetermined doses either by bathing during water hardening and incubation period or only during incubation period, and death rates were monitored during embryological development. The best larvae hatching rate was determined in 500 ppm oregano and 500 ppm laurel groups treated during water hardening plus daily as 82.11% and 79.87%, respectively. According to the results, it was determined that oregano and laurel essential oils exhibited better results in all doses compared with the negative control group, and 500 ppm dose had a better effect than the positive control group treated with formalin.