Bisphenols are known endocrine disruptors commonly utilized in food packaging and storage materials, which frequently come into touch with multiple food products packed in them. The bisphenols in fish feed and other feed materials for aquatic organisms are harmful. The consumption of such marine foods is hazardous. Hence, the feed of aquatic products needs to be verified for the presence of bisphenols. The present study was focused on developing and validating a rapid, selective, and sensitive method to quantify 11 bisphenols from the fish feed with dispersive solid-phase extraction, which was cleaned by an optimized amount of activated carbon spheres and silylated by N,O-bis(trimethylsilyl)trifluoro acetamide and analyzed by gas chromatography–mass spectrometry. The new method was rigorously tested and verified after carefully tuning various parameters affecting analyte recovery. Limit of detection (LOD) were set at 0.5–5 ng/g and limit of quantification (LOQ) at 1–10 ng/g, respectively, resulting in 95–114% recoveries. Interday and intraday precisions in terms of relative standard deviation were found to be less than 11%. The proposed approach was effectively applied in floating and sinking fish feeds. The obtained results showed that higher concentration of bisphenol A, followed by bisphenol TMC, and bisphenol M at a concentration of 256.10, 159.01, and 168.82 ng/g in floating feed and 88.04, 200.79, and 98.03 ng/g in sinking feed samples, respectively.

A rapid and sensitive analytical method was developed to quantitatively determine organic acids (OAs) from fish feed samples extracted by ion-pair (IP) solvent extraction, followed by in-situ butylation and gas chromatography-mass spectrometric (GC-MS) analysis. The extraction of OAs was carried out with acetonitrile containing 10 mM tetrabutylammonium hydroxide (TBAH), and the analytes were derivatized to their butyl esters in the injection port of the GC-MS system. The developed method was validated in the range of 1–5000 ng/g, with recoveries ranging from 93–117%. The limit of detection (LOD) and limit of quantification (LOQ) of the method was 1–5 ng/g and 2–10 ng/g, respectively, yielding good linearity (R2 > 0.9990) and precision with a relative standard deviation less than 10%. The proposed method was successfully applied to analyze OAs in sinking and floating fish feed samples. The analyzed samples showed the presence of benzoic, succinic, fumaric, glutaric, adipic, and phthalic acids in sinking feed samples; and benzoic, succinic, adipic, phthalic acids in floating feed samples, respectively.

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