No abstract

Objective: A simple, sensitive, rapid, precise and accurate stability indicating RP-HPLC method has been developed for simultaneous estimation of Hydroquinone, Hydrocortisone and Tretenoin from their Cream Formulation. Method: The Chromatographic separation was achieved on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 µm) column using a mobile phase consisting of Buffer (pH 4.0) 0.05M potassium dihydrogen ortho phosphate-Methanol in the ratio of 80:20% V/V at a flow rate of 1ml/min and UV detection at λmax 265 nm. The method showed linearity with correlation coefficient of Hydroquinone, Hydrocortisone and Tretenoin was 0.998, 0.998 and 0.996 over the range of 40-120 µg/ml, 20-60 µg/ml and 0.25-0.75 µg/ml respectively. Result: The retention time was 3 min, 5 min and 6 min for Hydroquinone, Hydrocortisone and Tretenoin respectively. The mean recoveries were found to be in the range of 97.00 –101.00 % for all the components. The method was validated as per the ICH guidelines. The method was validated as per the ICH guidelines. Conclusion: The method was stable and specific and when sample was stressed under different conditions like Acid, Base, Oxidative, Thermal and Photolytic, no interference of degradants were observed.

A simple, sensitive, rapid, precise and accurate RP-HPLC method has been developed for simultaneous estimation of Hydroquinone, Allantoin and Tretenoin from Cream Formulation. The Chromatographic separation was achieved on a reversed-phase InertsilC18 (4.6mm I.D. × 250mm, 5µm) column using a mobile phase consisting of Buffer (pH 3.5) 0.05M Potassium dihydrogen ortho phosphate-Methanol (70:30% V/V) at a flow rate of 1mL/min and UV detection at λmax 223 nm. The method showed linearity with correlation coefficient of Hydroquinone, Allantoin and Tretenoin was 0.999, 0.999 and 0.999 over the range of 100-300 µg/mL, 50-150 µg/mL and 0.625-1.875 µg/mL respectively. The mean recoveries were found to be 99.00 to 101.00 % for all the components. The method was validated as per the ICH guidelines. The method was stable and specific and when sample was stressed under different conditions like Acid, Base, Oxidative, Thermal and Photolytic, no interference of degradants were observed.

A simple, sensitive, rapid, precise and accurate RP-HPLC method has been developed for simultaneous estimation of hydroquinone, allantoin and tretinoin from cream formulation. Chromatographic separation was achieved on a reversed-phase Inertsil C18 (4.6 mm) I.D.× 250 mm, 5 µm) column using a mobile phase consisting of buffer (pH 3.5) 0.05 M potassium dihydrogen ortho phosphate-methanol (70:30% V/V) at a flow rate 1 mL min -1 and UV detection at λmax 223 nm. The method showed linearity with correlation coefficient of hydroquinone, allantoin and tretenoin being 0.999, 0.999 and 0.999 over the range of 100-300 µg mL-1, 50-150 µg mL-1 and 0.625-1.875 µg mL-1, respectively. The mean recoveries were found to be 99.00 to 101.00 % for all the components. The method was validated as per the ICH guidelines. The method was stable and specific and when sample was stressed under different conditions like acid, base, oxidative, thermal and photolytic, no interference of degradants were observed.