Objective: A new, simple, sensitive and economical UV spectrophotometric method was developed for the simultaneous analysis of Sulfadiazine [SDA] and Trimethoprim [TMP] in pharmaceutical formulations.Methods: This UV method was developed with methanol as solvent. The wavelengths selected for analysis in the present method were 265 nm for TMP and 289 nm for SDA. Teccomp UV-2301 double beam UV/Vis spectrophotometer was used to carry out spectral analysis and the data was recorded by Hitachi software.Results: Linearity was found to be within the concentration range of 2-9 µg/ml TMP and 9.08-41 µg/ml of SDA. Accuracy of the method was determined by recovery studies. Percentage recovery was found to be 98.20-99.25 for TMP with a % RSD of 0.338, 0.506 and 0.510 for three spiked levels. % RSD was found to be 0.229 and 0.380; 0.212 and 0.328 for SDA, TMP in intra and inter-day precision respectively. The % RSD value in ruggedness was found to be 0.440 for SDA and 0.569 for TMP. Conclusion:The advantages of this method for analytical purposes lie in the rapid determination, its cost-effectiveness, easy preparation of the sample, good reproducibility. In addition to this, the present method can be recommended for simultaneous determination of SDA and TMP in routine quality control analysis in combined drug formulations.