There is a need to scientifically validate the claim that wild species of medicinal plants are more potent than the cultivated plants. Thus, this study evaluated the phytochemical and antioxidant properties of wild and cultivated Alepidea amatymbica. Acetone, methanol, and water extracts of the rhizome of wild and cultivated A. amatymbica were evaluated for total phenol, flavonol, flavonoid, tannin, proanthocyanidin, saponin, and alkaloid contents using spectrophotometric methods. In vitro antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), nitric oxide (NO), and hydrogen peroxide assays. The wild extracts had higher phytochemical contents in most of the assays than cultivated extracts. Total phenol in the wild extracts ranged from 32.30 to 117.8 mg GAE/g with the acetone extracts having the highest content while the water extracts were the least. The range in the total phenol of the cultivated species was 66.46 to 98.44 mg GAE/g with the methanol extracts having the highest content while water extracts was the least. The flavonoid content ranged from 55.01 to 99.09 mg QE/g and from 48.65 to 67.32 mg QE/g for the wild and cultivated plants, respectively. The alkaloid contents ranged from 14.70 to 17.80% in the wild species while it ranged from 11.98 to 13.21% in the cultivated species. The wild species also showed higher antioxidant activities in most of the assays evaluated. This study has implications for both pharmacological and conservation purposes.

Alepidea amatymbica is used as a herbal medicine for the treatment of various diseases. As a result of its high medicinal value, this plant is being overexploited by herbal traders with little attention being paid to its conservation, which could lead to its extinction. Cultivation of Alepidea amatymbica was conducted to determine the appropriate planting depth and rhizome fragment length for the growth of this plant. The experiment was laid out in a Complete Randomized Block Design (CRBD) with two factors in a 6 × 3 factorial design. There were six levels of fragment length (1, 2, 3, 4, 5 and 6 cm) and three levels of burial depth (2.5, 5 and 7.5 cm). Emergence rate, number of leaves, leaf area, and plant height, number of florets, rhizome length gain, rhizome weight gain, shoot moisture, and rhizome moisture were measured as growth parameters. The best overall yield in terms of plant height, shoot emergence, rhizome weight gain, number of florets and number of leaves was observed in 7.5 cm planting depth at 6 cm rhizome length. Four- centimetre rhizome length had the highest leaf area of 111.9 ± 3.5 cm2, 101.3 ± 3.5 cm2, 105 ± 3.5 cm2 at 2.5, 5, 7.5 cm planting depth respectively. Shorter fragment lengths showed high potential for vegetative propagation in terms of rhizome length gain at all burial depths. These results suggest that A. amatymbica can regenerate from buried rhizomes and they may contribute to the establishment of a protocol for propagation that could help in conservation of this plant to avoid its extinction