Myristica fragrans, also known as nutmeg is a spice that cures various diseases. This study aimed to synthesize silver nanoparticles from a hydroethanolic extract of Myristica fragrans seeds (MFHE) and evaluate their anti-diabetic properties. To MFHE, AgNO 3 solution was added and exposed to sunlight to produce silver nanoparticles from hydroethanolic seed extract of Myristica fragrans (MFHENP). The MFHENP was characterized by numerous techniques. UV-visible spectroscopy con rmed the formation of silver nanoparticles by the absorption peak at 430nm. Scanning electron microscopy (SEM) studies revealed the shape and size of the particles at the range of 50-60nm. Energy-dispersive X-ray spectroscopy (EDX) disclosed the presence of silver ions. X-ray diffraction spectrum con rmed the crystalline nature of silver nanoparticles by the peak at 39 o . FTIR analysis revealed the functional groups present in MFHE as well as in MFHENP and Zeta potential analysis was found to be 14mV. Furthermore, in-vitro anti-diabetic activity was investigated. MFHENP showed signi cant e ciency against the inhibition of alpha-amylase and alpha-glucosidase enzymes and also MFHENP retarded the glucose transport across the membrane which is analyzed by glucose diffusion and glucose uptake assays.Acarbose is used as a standard for all these methods and MFHENP e ciency proves their therapeutic potential for the treatment of diabetes mellitus.