A large number of epidemiological studies have evaluated the association between Human papillomavirus infection and breast carcinoma, but the results have been inconsistent.Numerous types of cytopathic effect are distinct in living cultures, but fixation and staining of the cells is essential to realize appearances of viral infection such as inclusion bodies. Identifying cytopathic effect and using it as a investigative tool needs much practice in observing stained section of numerous cell types. The present study aimed at diagnosing the histological changes in the malignant breast tissue, which caused by the HPV and comparing them with malignant breast tissue caused by other factors in order to find a way to diagnose the virus through the histopathic effect. A total of 100 samples was included in the study, 20 from benign tumor and 80 carcinomas, including different histopathological change. HPV was detected by using the conventional PCR. The breast cancer sections were examined for the expression of the effect of Human papillomavirus. In separate series the results of using formalin-fixed, paraffin -embedded sections were compared. Overall prevalence of HPV in malignant breast tissue was difference, we found that the histopathological characteristics of HPVassociated breast cancer are very different to breast cancercaused by other factors. Specifically, we showed the presence of HPV in breast cancer associates with the adenocarcinoma, The virus caused more activity in the tumor. Tumor is acinar carcinoma with increased cellularty and proliferative, While, in none infected with virus, there is no increased in cellularty or acinar, in some arease there was dilution of ducts.The observations indicate a likely causal role of HPV in causing a different histopathological change than breast cancercaused by other factors, This helps in the diagnosing of the HPV through cytopathic effect.

This research is aimed at investigating the hepatotoxic effect of hydrogen peroxide and effects of Curcuma longa and zingiber officinale on this hepatotoxicity. In present study we administration hydrogen peroxide to animals rat for induced high cholesterol in blood and caused liver damage . These were carried out via estimation of liver function tests and histochange in the liver. Fourteen adult Westar rats Rattus norvegicus were used for the experimental investigations. They were grouped into four groups:. Animals in group one served as the control group. Group two consists of rats administered with hydrogen peroxide (1% body weight) .Group three consists of rats administered with hydrogen peroxide and Curcuma longa (200 mg/ kg body weight).Group four administered with hydrogen peroxide and zingiber officinale(200 mg/ kg body weight). All animals administration orally, the experimental period lasted for 30 days. The rats were sacrificed on the thirteen day. The blood samples collected by cardiac puncture and placed in appropriately labeled bottles for the various assays. And section of liver isolated. There was a decrease in the aspartate aminotransaminase level, alanine aminotransferase level are significantly lower (P<0.05) in the rats of the control group than both the rats treated with hydrogen peroxide only and the ones treated peroxide with Curcuma longa and peroxide with zingiber officinale These were lower in the rats treated with Curcuma longa and zingiber officinale than the rats treated with hydrogen peroxide alone. These reduction is however not statistically significant and it presents that these could be dosage related. The histological change in the section of liver showed as clusters of inflammatory cells surrounding portal area

Objective The effects of camphor in the histological change on some organs of female rats. In Iraq, camphor in the form of tablets was added to wash dead bodies, and thus putting washers in great risk, especially female washers. Though, the literature concerning its histological toxicity has not been documented. It has long been used in dead bodies washed. In our study, we used high dose of camphor to investigate the effect of camphor in histological change on some organs. Methods The rats were divided into three groups (n = 6), the first group (A) was a control group and treated with distilled water, the second and third groups (B and C) treated with 50 mg camphor/kg and 75 mg camphor/kg, respectively. At the end of the first month of treatment, animals were anesthetized; liver, kidney, spleen, and ovary were removed for serial sections, and histological staining.Results The results showed vaculation in hepatocytes, and infiltration of inflammatory cells loss of most of the architecture and cell boundaries in liver of group (B), while in group (C), there was congestion in area of Portia, detachment of some hepatocytes, and inflammatory cells. Kidney of group B showed edematous glomerulus, glomerular lobulation, and increased number of nuclei. Also, there were swollen glomerulus, increase number of nuclei, and congestion of blood cells in kidney of group C. Spleen of groups B and C revealed non-active lymphoid tissue and degenerated lymphocytes cells, congestion in lymphoid tissue, and depletion of white pulp mainly neutrophils and macrophage. Also, the results showed congestion in follicles (B) and hemorrhage (C), detachment in oocytes in treatment groups compared with control group. Conclusions The oral administration of varying concentration of camphor solution to the animal rat has a cytotoxic effect on organs, such as the liver, kidney, spleen, and ovary.

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Metformin is recommend as the first line oral agent to be used patients with non-insulin-dependent diabetes mellitus(Type 2 Diabetic).The study was prospectively performed in diabetic and endocrine center of Nassiriyah city .The present study aimed to shed light on metformin and hemoglobin(Hb) and lipid profile in type II diabetic patients with diet and without diet. Our study included 60 patients treated with metformin for Twelve-week. The subjects were divided into three groups. Group I as control, group II patients received Metformin-(1000/2 mg/day) with diet and group III patients received the Metformin-(1000/2 mg/day) without diet all these patient had been selected from those patients visit. The Hb concentration and lipid profile were been examine after 3 months of treatment.In present study we found the hemoglobin concentration reduced in group II with diet patient compare with the group III and control group also reduced in the group III without diet patient compare with control group, as same time This study showed that the treatment with metformine led to, reductions observed in the cholesterol concentrations of the group II diabetic patients with diet compared with the group III administered MET respective without diet diabetic and compared with control, Also we found the triglyceride concentrations of diabetic patients in group administered MET respective with diet were significantly (p<0.05) reduced compared with that of without diet diabetic and control therefore it was considered predisposing factor to reduced damage diabetes. For this reason; continuous taking of metaformin should be important in medicine and general healthy, and they must be not used unless consult of specialists.