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We report the molecular cloning of a proline/arginine-rich protein (called PARP) from human cartilage using the polymerase chain reaction (PCR) and degenerate oligonucleotides based on the previously published amino acid sequence of bovine PARP [l]. Subsequently, a reverse transcriptionpolymerase chain reaction (RT-PCR) was performed with poly(A)-rich RNA from human cartilage using a sense oligonucleotide derived from PARP and an anti-sense oligonucleotide derived from the known sequence of the human collagen a2(XI) chain [2]. Nucleotide sequencing of the PCR product demonstrated that PARP is a fragment of the NHz-terminal non-collagenous (NC3) domain of the collagen a2(XI) chain.

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