Background: Bacteremia caused by non-fermenting gram negative bacilli (NFGNB) is a serious infection which is associated with high morbidity and mortality. Identification of NFGNB and monitoring their susceptibility pattern are important for proper management of these infections. Aim: Scope of the present study is to characterize the NFGNB along with their antimicrobial sensitivity pattern among the patients coming to our hospital, a tertiary care center. Materials & Methods: A retrospective study was conducted to determine the NFGNB isolated from blood during the one-year study period. A total of 8223 Blood cultures were received in the laboratory which was processed by semi-automated technique in Bac-T Alert. Antibiotic susceptibility was performed by VITEK and Kirby Bauer methods and interpreted by CLSI guidelines. 102 isolates of NFGNB were obtained from the Blood culture. Results: Most commonly isolated organism is Acinetobacter species (47%) followed by Pseudomonas spp (27%) and Burkholderia spp(15.6%). Maximum isolates of Acinetobacter spp were sensitive to colistin (91.9%), cefoperazone-sulbactum (88.4%), Tigecycline (62.5%). Pseudomonas spp was sensitive to colistin (100%), ciprofloxacin (59%). Conclusion: Identification of NFGNB and monitoring their susceptibility patterns will help in proper management of infections caused by them.