Background: Bacteremia caused by non-fermenting gram negative bacilli (NFGNB) is a serious infection which is associated with high morbidity and mortality. Identification of NFGNB and monitoring their susceptibility pattern are important for proper management of these infections. Aim: Scope of the present study is to characterize the NFGNB along with their antimicrobial sensitivity pattern among the patients coming to our hospital, a tertiary care center. Materials & Methods: A retrospective study was conducted to determine the NFGNB isolated from blood during the one-year study period. A total of 8223 Blood cultures were received in the laboratory which was processed by semi-automated technique in Bac-T Alert. Antibiotic susceptibility was performed by VITEK and Kirby Bauer methods and interpreted by CLSI guidelines. 102 isolates of NFGNB were obtained from the Blood culture. Results: Most commonly isolated organism is Acinetobacter species (47%) followed by Pseudomonas spp (27%) and Burkholderia spp(15.6%). Maximum isolates of Acinetobacter spp were sensitive to colistin (91.9%), cefoperazone-sulbactum (88.4%), Tigecycline (62.5%). Pseudomonas spp was sensitive to colistin (100%), ciprofloxacin (59%). Conclusion: Identification of NFGNB and monitoring their susceptibility patterns will help in proper management of infections caused by them.

Introduction: Diabetic foot is an important and devastating complication of the Type II diabetes mellitus. This study was mainly undertaken to study the combination of NPWT along with topical insulin application and its effect on wound healing when compared to NPWT alone. Material and Methods: A prospective interventional study was undertaken in the Department of Surgery, Bengaluru. A total of 100 patients were divided equally between the two groups where one group received NPWT alone and another group received NPWT with topical insulin. The wounds in both the experimental and control groups were opened on the 7th, 15th and 30th day and compared using the BATES - JENSEN WOUND ASSESSMENT TOOL. The details were collected by using a predesigned proforma and analysed using Statistical Package for Social Services (SPSS ver 20). Results: Majority of the patients in both experimental and control groups were aged more than 60 years and were males. There was no statistically significant difference between the NPWT alone and NPWT with topical insulin groups.