A saúde única integra as esferas da saúde humana, ambiental e animal, por meio de políticas públicas focadas na prevenção e controle de enfermidades infecciosas. O médico veterinário é um profissional atuante na área da saúde pública pelo contato direto com as três esferas, e uma das principais ações desse profissional inclui a promoção de medidas comunitárias educativas para disseminar informações em relação à prevenção de doenças zoonóticas. Diante da atual pandemia, desenvolvemos o projeto: “Efeito borboleta aplicado a asilos de Uberlândia-MG no combate à COVID-19” com o objetivo de divulgar informações sobre a doença de maneira lúdica, e atender as demandas das instituições envolvidas. Foram produzidos e distribuídos dez materiais didáticos como vídeos e jogos interativos com orientações em relação à COVID-19 em 16 abrigos de idosos, junto à arrecadação de produtos de limpeza e higiene pessoal, máscaras e gêneros alimentícios, para enfatizar as medidas preventivas, provenientes de doações de 23 empresas. Funcionários das instituições desenvolveram as atividades propostas com os idosos, ressaltando a importância do distanciamento social e da higiene pessoal no momento atual. A ação executada permitiu a ampliação do conhecimento a respeito da pandemia, em virtude do uso de metodologias aplicadas às dificuldades e necessidades dos idosos, aliada à motivação emocional utilizada na proposta, o que favoreceu o processo ensino-aprendizagem. A presente ação social nas instituições auxiliou na conscientização e esclarecimento de dúvidas em relação à COVID-19 ao principal grupo de risco, e reforçou o contexto de atuação do médico veterinário na saúde única. Palavras-chave: Educação; Epidemia; Grupo vulnerável; Promoção de saúde Veterinary medicine and the one health system: socio-educational action applied to elderly in the context of COVID-19 Abstract: The one health system integrates the human, environmental, and animal health spheres through public policies focused on preventing and controlling infectious diseases. The veterinary doctor is a professional actor in public health through direct contact with the three spheres. One of these professional's main actions includes promoting community educational measures to disseminate information regarding the prevention of zoonotic diseases. In the face of the current pandemic, we developed the project: "Butterfly effect applied to asylums of Uberlândia, Minas Gerais State, Brazil, in the combat to COVID-19" to spread information about the disease playfully and to attend to the demands of the involved institutions. Ten educational materials were produced and distributed, such as videos and interactive games with guidelines regarding COVID-19, in 16 shelters for the elderly, along with the collection of cleaning and personal hygiene products, masks, and food products from donations from 23 companies, to emphasize preventive measures. Institutional staff developed the proposed activities with the elderly, emphasizing the importance of social distance and personal hygiene at this time. The action performed allowed the amplification of knowledge about the pandemic due to the use of methodologies applied to the difficulties and needs of the elderly, allied to the emotional motivation used in the proposal, which favored the teaching-learning process. This social action in the institutions helped to raise awareness and clarify doubts concerning VOCID-19 to the leading risk group and reinforced the context of the veterinarian's action in the one health. Keywords: Education; Epidemic; Vulnerable group; Health promotion

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The presence of virulence genes, phylogenetic relationships, biofilm formation index (BFI), and ultrastructure in S. Minnesota at different temperatures (4, 25, and 36 °C) were analyzed. In addition, the ability of biocidal agents (chlorhexidine1%, sodium hypochlorite 1%, and peracetic acid 0.8%) to inhibit biofilms formed by 20 strains isolated from broiler slaughter plants from two Brazilian companies in 2009, 2010, and 2014 was determined. The presence of specific genes was evaluated by PCR and phylogeny between strains by pulsed-field gel electrophoresis. The BFI was determined using tryptone soy broth with 5% of chicken juice, and its structure was observed by scanning electron microscopy. The presence of specific genes indicated that S. Minnesota has the potential to cause disease in humans, adapting to adverse conditions. Temperatures of 25 and 36 °C favored biofilm formation, although at 4 °C, there was still biomass that could contaminate the final product. Tolerance to all biocides was identified in 12/20 (60%), representing a real risk of adaptation mechanisms development, especially regarding to resistance to sodium hypochlorite. Phylogenetic analysis indicated cross-contamination and spread among companies, which was probably related to biofilms formation. Results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite, including the need for rigorous control, adopting low temperatures to prevent biofilms formation in the poultry industry.

Despite being considered fragile and fastidious, Campylobacter jejuni is the most prevalent cause of foodborne bacterial gastroenteritis, and chicken meat is considered the main vehicle of transmission to humans. This agent can survive adverse conditions in the form of biofilms, but extreme stress (nutritional, oxidative and thermal) promotes the acquisition of a state called viable but not culturable (VBNC). The emergence of this pathogen worldwide and the recent international requirements in its control instigated us to qualitatively and quantitatively estimate the time required for the acquisition of the VBNC form in 27 strains of C. jejuni, characterize morphological aspects, determine its adaptive and invasive potential and perform comparative metabolomic evaluation. Extreme stress promoted the complete acquisition of the VBNC form in a mean time of 26 days. Starting from an average initial count of 7.8 log CFU/mL, the first four days determined the greatest average reduction of the culturable form of 3.2 log CFU/mL. The scanning and transmission image analyses showed a transition from the typical viable form (VT) to the VBNC form, with initial acquisition of the straight rod shape, followed by loss of the flagella and subdivision into two to 11 imperfect cocci arranged in a chain and rich in cellular content, until their individual release. RT-PCR identified the presence of ciaB and p19 transcripts in the 27 cultivable C. jejuni strains, a character maintained in the VBNC form only for p19 and in 59.3% (16/27) of the VBNC strains for the ciaB gene. The average inoculation of 1.8 log CFU/mL of C. jejuni VBNC into primary chicken embryo hepatocyte cells promoted the occurrence of apoptosis processes significantly after 24 hours of contact by one of the strains tested. In C. jejuni VBNC, we detected higher expression of metabolites linked to protective and adaptation mechanisms and of volatile organic precursor compounds indicative of metabolism interruption. The oscillations in the time of acquisition of the VBNC form together with the presence of transcripts for ciaB and p19, the identification of cell lysis and metabolites that ensure the maintenance of the pathogen alert to the fact that C. jejuni VBNC remains virulent and adapted to stress, which makes evident the potential danger of this latent form, which is not detectable by official methodologies.