Genotypical Relationship Between Human and Poultry Strains of Campylobacter jejuni

Melo, Roberta Torres de; Dumont, Carolyne Ferreira; Braz, Raquelline Figueiredo; Monteiro, Guilherme Paz; Takeuchi, Micaela Guidotti; Lourenzatto, Eduarda Cristina Alves; Santos, Jandra Pacheco dos; Rossi, Daise Aparecida

doi:10.1007/s00284-021-02553-2

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…The cadF gene is considered a virulence factor and core genomic feature, but virulence profiling studies of C. jejuni /C. coli strains including several from South America report the absence of the cadF gene within C. jejuni / C. coli strains using PCR based detection [21][22][23]. Furthermore, BLASTn analysis of WGS contigs at 90% identity level over 90% of the gene length can also result in failure to detect the cadF gene, yet the presence of the cadF gene in all complete genomes of C. jejuni / C. coli supports its current identity as a core gene.…”

Section: Discussionmentioning

confidence: 99%

“Candidatus Campylobacter infans” detection is not associated with diarrhea in children under the age of 2 in Peru

et al. 2022

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A working hypothesis is that less common species of Campylobacter (other than C. jejuni and C. coli) play a role in enteric disease among children in low resource settings and explain the gap between the detection of Campylobacter using culture and culture independent methods. “Candidatus Campylobacter infans” (C. infans), was recently detected in stool samples from children and hypothesized to play a role in Campylobacter epidemiology in low- and middle-income countries (LMIC). This study determined the prevalence of C. infans in symptomatic and asymptomatic stool samples from children living in Iquitos, Peru. Stool samples from 215 children with diarrhea and 50 stool samples from children without diarrhea under the age of two were evaluated using a multiplex qPCR assay to detect Campylobacter spp. (16S rRNA), Campylobacter jejuni / Campylobacter coli (cadF gene), C. infans (lpxA), and Shigella spp. (ipaH). C. infans was detected in 7.9% (17/215) symptomatic samples and 4.0% (2/50) asymptomatic samples. The association between diarrhea and the presence of these targets was evaluated using univariate logistic regressions. C. infans was not associated with diarrhea. Fifty-one percent (75/146) of Campylobacter positive fecal samples were negative for C. jejuni, C. coli, and C. infans via qPCR. Shotgun metagenomics confirmed the presence of C. infans among 13 out of 14 positive C. infans positive stool samples. C infans explained only 20.7% of the diagnostic gap in stools from children with diarrhea and 16.7% of the gap in children without diarrhea. We posit that poor cadF primer performance better explains the observed gap than the prevalence of atypical non-C. jejuni/coli species.

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Section: Discussionmentioning

confidence: 99%

“Candidatus Campylobacter infans” detection is not associated with diarrhea in children under the age of 2 in Peru

et al. 2022

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“…A previous study has evaluated the genotypic relationship between human and poultry strains of C. jejuni in Brazil and observed that human isolates presented limited virulence capacity when compared to their poultry counterparts, and were different in molecular typing. They also observed that the ability to cause GBS was similar for both strains [41].…”

Section: Discussionmentioning

confidence: 83%

“…On the contrary, although the human-derived strains were also obtained from a single institution, they were isolated from human samples received from across the country. However, it is noteworthy that previous studies have found that, despite the evidence that virulenceassociated genes in C. jejuni are widely dispersed in both species, poultry isolates presented a high occurrence of virulence markers [10,41,42]. A previous study has evaluated the genotypic relationship between human and poultry strains of C. jejuni in Brazil and observed that human isolates presented limited virulence capacity when compared to their poultry counterparts, and were different in molecular typing.…”

Section: Discussionmentioning

confidence: 99%

The Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources

Lima

Perdoncini

Borges

et al. 2022

J Infect Dev Ctries

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Introduction: Campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis. Despite its public health importance, the virulence factors and mechanisms underlying C. jejuni pathogenesis remain poorly understood and the relationships between these genes and the sources of the strains are not clear. We aimed to determine the virulence profiles of C. jejuni isolated from poultry and human cases of Campylobacteriosis. Methodology: A total of 50 strains of C. jejuni isolated from poultry and human cases of Campylobacteriosis were screened by polymerase chain reaction (PCR) for the presence of six pathogenic genes (flaA, iam, wlaN, cdtA, cdtB, cdtC). Results: A total of 40% (10/25) of the human isolates presented only one virulence marker. In contrast, 64% (16/25) of the poultry-derived strains showed four or five virulence markers. cdtA and flaA occurred more frequently in poultry-derived strains than in human strains. Ten different virulence profiles were observed among the human isolates and 11 among the poultry strains. Only four profiles were common to both sources: profiles 3 (flaA, cdtA, cdtB, and cdtC), 5 (cdtA and cdtB), 7 (flaA and cdtB), and 10 (iam, flaA, cdtA, cdtB, and cdtC). The human-derived strains had a higher Shannon diversity index (1.9396) and Simpson index (0.8367), indicating a more diversified population than found in poultry (1.7742 and 0.7333, respectively). Conclusions: We found variations in the genetic profiles of the circulating strains based on the isolation source and genes that are potentially pathogenic to humans were detected in poultry-derived strains.

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“…The cadF, pldA and ciaB genes showed higher prevalence among the isolates obtained from chicken carcasses. Moreover, the ctdABC and luxS genes showed low prevalence among the clinical isolates, showing their presence in only 15% and 45% of human C. jejuni genomes [63].…”

Section: Virulence and Antibiotic Resistance Of Campylobacter Spp Pou...mentioning

confidence: 98%

Overview of Virulence and Antibiotic Resistance in Campylobacter spp. Livestock Isolates

Bunduruș¹,

Balta²,

Ştef³

et al. 2023

Antibiotics

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Campylobacter remains the most prevalent foodborne pathogen bacterium responsible for causing gastroenteritis worldwide. Specifically, this pathogen colonises a ubiquitous range of environments, from poultry, companion pets and livestock animals to humans. The bacterium is uniquely adaptable to various niches, leading to complicated gastroenteritis and, in some cases, difficult to treat due to elevated resistance to certain antibiotics. This increased resistance is currently detected via genomic, clinical or epidemiological studies, with the results highlighting worrying multi-drug resistant (MDR) profiles in many food and clinical isolates. The Campylobacter genome encodes a rich inventory of virulence factors offering the bacterium the ability to influence host immune defences, survive antimicrobials, form biofilms and ultimately boost its infection-inducing potential. The virulence traits responsible for inducing clinical signs are not sufficiently defined because several populations have ample virulence genes with physiological functions that reflect their pathogenicity differences as well as a complement of antimicrobial resistance (AMR) systems. Therefore, exhaustive knowledge of the virulence factors associated with Campylobacter is crucial for collecting molecular insights into the infectivity processes, which could pave the way for new therapeutical targets to combat and control the infection and mitigate the spread of MDR bacteria. This review provides an overview of the spread and prevalence of genetic determinants associated with virulence and antibiotic resistance from studies performed on livestock animals. In addition, we have investigated the relevant coincidental associations between the prevalence of the genes responsible for pathogenic virulence, horizontal gene transfer (HGT) and transmissibility of highly pathogenic Campylobacter strains.

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Genotypical Relationship Between Human and Poultry Strains of Campylobacter jejuni

Cited by 6 publications

References 29 publications

“Candidatus Campylobacter infans” detection is not associated with diarrhea in children under the age of 2 in Peru

“Candidatus Campylobacter infans” detection is not associated with diarrhea in children under the age of 2 in Peru

The Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources

Overview of Virulence and Antibiotic Resistance in Campylobacter spp. Livestock Isolates

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