Introduction: The use of plant extracts in the green synthesis of metallic nanoparticles is one of the simplest, most practical, economical, and ecologically friendly methods for avoiding the use of toxic chemicals.Method: Silver nanoparticles (AgNPs) were synthesized, employing a high-efficiency, non- toxic, cost-effective, green, and simple technique that included the use of Salacia oblonga root extract (SOR) as a capping agent compared to synthetic nanoparticles. The use of S. oblonga can be seen in traditional medicines for treating diabetes, obesity, rheumatism, gonorrhea, asthma, and hyperglycemia. The objectives of the current study were to green synthesize S. oblonga root extract silver nanoparticles (SOR-AgNPs), characterize them, and study their antioxidant, antibacterial, and antidiabetic activities.Result: The shape of SOR-AgNPs was spherical, at less than 99.8 nm in size, and exhibited a crystalline peak at XRD. The green synthesized SOR-AgNPs showed significant antioxidant properties like DPPH (80.64 μg/mL), reducing power capacity (81.09 ± SEM μg/mL), nitric oxide (96.58 μg/mL), and hydroxyl (58.38 μg/mL) radical scavenging activities. The MIC of SOR-AgNPs was lower in gram-positive bacteria. The SOR-AgNPs have displayed efficient inhibitory activity against α-amylase, with an EC50 of 58.38 μg/mL. Analysis of capping protein around the SOR-AgNPs showed a molecular weight of 30 kDa.Discussion: These SOR-AgNPs could be used as antibacterial and antidiabetic drugs in the future as it is cheap, non-toxic, and environmentally friendly. Bio-fabricated AgNPs had a significant impact on bacterial strains and could be used as a starting point for future antibacterial drug development.

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The aim of the present study was to perform preliminary phytochemical screening and methanol and ethyl acetate seed extracts of such as Tribulus terrestris (Tt) and Solanum torvum (Sn) through FT-IR spectroscopy method. Material and Methods: The methanol, ethyl acetate extract was screened for its potential antioxidant activity by phenols, tannins, flavonoids and FT-IR spectroscopic studies revealed different characteristic peak values with various functional compounds. Results: The total phenolic content of the methanol, ethyl acetate Tt extract was (2.71 ±0.17, 2.32 ± 0.18) mg gallic acid equivalent (GAE)/g of extract. The total flavonoid and tannin content (2.15 ± 0.15, 1.98 ± 0.07) mg quercetin equivalent/g of extract and (2.35 ±0.12, 1.75 ±0.06) mg GAE/g of the extract. The total phenolic content of the methanol, ethyl acetate Sn extract was (1.63 ± 0.08, 2.28 ±0.18) mg gallic acid equivalent (GAE)/g of extract. The total flavonoid and tannin content (0.96 ± 0.06, 1.56 ± 0.19) mg quercetin equivalent/g of extract and (1.92 ± 0.09, 2.63 ± 0.13) mg GAE/g of the extract. The FT-IR method was performed on a spectrophotometer system, which was used to detect the characteristic peak values and their functional groups respectively. Conclusion: The results of the present study preliminary phytochemical screening generated the FT-IR spectrum profile for Tt and Sn. In conclusion, the obtained results of the tests demonstrated that this plant might be used in the prevention and in the treatment of different diseases related to oxidative stress.