Peternakan sapi rakyat merupakan salah satu penopang ekonomi di Provinsi Lampung namun kondisi tatalaksana manajemen reproduksi dan kesehatannya belum optimal sehingga terjadi gangguan reproduksi dan kesehatan ternak. Desa Purworejo, Kecamatan Negeri Katon, Kabupaten Pesawaran, Provinsi Lampung merupakan salah satu desa yang masyarakatnya banyak memelihara sapi. Berdasarkan survei pendahuluan tampak bahwa pengetahuan dan wawasan peternak tentang manajemen reproduksi dan kesehatan sehingga produktivitas ternak sapi masih rendah. Tim Pengabdian Kepada Masyarakat melalui penyuluhan terkait manajemen reproduksi dan kesehatan sapi bertujuan meningkatkan pengetahuan, pemahaman serta cara pemeliharaan ternak rakyat secara konvensional menjadi berbasis ilmu pengetahuan dan meningkatkan kesehatan ternak melalui pengobatan massal ternak. Sasaran kegiatan pengabdian ini yaitu masyarakat peternak sapi di Desa Purworejo Kecamatan Negeri Katon Kabupaten Pesawaran. Kegiatan pengabdian ini dilakukan dengan metode 1) Penyuluhan meliputi kegiatan penyuluhan dan diskusi mengenai manajemen reproduks dan kesehatan; dan 2) Pemeriksaan kesehatan dan pengobatan massal ternak sapi. Evaluasi kegiatan dilakukan untuk mengetahui sejauh mana respon peserta penyuluhan terhadap pelaksanaan kegiatan pengabdian dengan cara membandingkan perubahan nilai pada evaluasi awal (pre-test) dan evaluasi akhir (post-test). Kesimpulan kegiatan ini yaitu 1) kegiatan penyuluhan diikuti oleh 14 peternak dengan pemeriksaan kesehatan dan pengobatan massal dilakukan pada 23 ekor sapi; dan 2) kegiatan pengabdian masyarakat meningkatkan pengetahuan dan kemampuan peternak dengan skor tinggi pada sisi pengetahuan kesehatan ternak sapi sebesar 86,43%; sisi reproduksi sebesar 82,86%; dan sisi pengetahuan sanitasi kandang sapi sebesar 91,27%.

Aim:Avian encephalomyelitis (AE) is a viral disease which can infect various types of poultry, especially chicken. In Indonesia, the incidence of AE infection in chicken has been reported since 2009, the AE incidence tends to increase from year to year. The objective of this study was to analyze viral protein 2 (VP-2) encoding gene of AE virus (AEV) from various species of birds in field specimen by reverse transcription polymerase chain reaction (RT-PCR) amplification using specific nucleotides primer for confirmation of AE diagnosis.Materials and Methods:A total of 13 AEV samples are isolated from various species of poultry which are serologically diagnosed infected by AEV from some areas in central Java, Indonesia. Research stage consists of virus samples collection from field specimens, extraction of AEV RNA, amplification of VP-2 protein encoding gene by RT-PCR, separation of RT-PCR product by agarose gel electrophoresis, DNA sequencing and data analysis.Results:Amplification products of the VP-2 encoding gene of AEV by RT-PCR methods of various types of poultry from field specimens showed a positive results on sample code 499/4/12 which generated DNA fragment in the size of 619 bp. Sensitivity test of RT-PCR amplification showed that the minimum concentration of RNA template is 127.75 ng/µl. The multiple alignments of DNA sequencing product indicated that positive sample with code 499/4/12 has 92% nucleotide homology compared with AEV with accession number AV1775/07 and 85% nucleotide homology with accession number ZCHP2/0912695 from Genbank database. Analysis of VP-2 gene sequence showed that it found 46 nucleotides difference between isolate 499/4/12 compared with accession number AV1775/07 and 93 nucleotides different with accession number ZCHP2/0912695.Conclusions:Analyses of the VP-2 encoding gene of AEV with RT-PCR method from 13 samples from field specimen generated the DNA fragment in the size of 619 bp from one sample with sample code 499/4/12. The sensitivity rate of RT-PCR is to amplify the VP-2 gene of AEV until 127.75 ng/µl of RNA template. Compared to Genbank databases, isolate 499/4/12 has 85% and 92% nucleotide homology.

Province of Lampung has a strategic opportunity for the development of ruminants because of the availability of natural resources that support it. One type of ruminant livestock is goat. People's goat farming is now widespread but the conditions of maintenance management are not optimal. Constraints that are still often faced by goat farmers are in terms of lack of knowledge on handling reproductive disorders and livestock health, cage sanitation that is not in accordance with regional geographical conditions and the lack of assistance for livestock treatment obtained. The Community Service Team Scheme Beginner Lecturers of BLU University of Lampung 2020 through counseling related to health management, reproduction and pen sanitation as well as mass treatment of goats were aims to increase knowledge and how to raise conventional people's livestock to be science-based. The target of the activity was goat breeders in Kota Agung Village, South Sungkai District, North Lampung Regency. Methods of activity, namely 1) Extension of health management, reproduction, and sanitation for goat sheds; 2) Implementing a pen sanitation program with cleaning and disinfection of the pen; and 3) Health checks and mass treatment. Activity measurement parameters to determine the response of extension participants to the implementation of community service activities by comparing changes in values in the initial evaluation (pre-test) and the final evaluation (post-test). Service activities were 1) 31 goat farmers was attending the counseling; 2) Mass treatment activities were carried out on 232 goats; and 3) Pen sanitation activities were carried out at 31 goat pen with the results of the evaluation of activities, namely the level of breeders' knowledge on the goat health knowledge increased by 54.02%; the reproductive knowledge increased by 26.77%; and the pen sanitation knowledge increased by 52.33% after the activities were implemented. Conclusion of the overall activities had a very large positive impact on farmers.

The aim of this study was analyzed the effort to implement the integration of aquaponic technology in conventional fish cultivation. The study was conducted for 6 months (May - October 2021) in the Mandiri Sentosa fish farming unit in Marga Agung Village, Jati Agung District, South Lampung Regency. Experimental research with quantitative methods with descriptive analysis of two treatments is the analysis of the feasibility of catfish cultivation business that has not been integrated with Aquaponik technology (P0) and which has been integrated with Aquaponic technology (P1). Each treatment pool with a 2,000 catfish density with 5-7 cm/fish marked size was maintained for 3 months for harvest. The integration of aquaponic technology in the treatment pool (P1) with the number of vegetable seeds of lettuce was 100 seeds per treatment pool. Quantitative method was doing by business analysis to determine the activities of catfish cultivation business (Clarias sp.) Has benefits and deserves to be developed from the parameters of business cost analysis, depreciation, acceptance analysis, net cash flow (net cash flow), R/C ratio, B/C ratio, Break-Even Point (BEP), Payback Period. The conclusion of this research was the farming business based on aquaponic technology in Catfish (Clarias sp.) cultivation was profitable business or very suitable to be developed.

Background and Objective: The growth hormone (GH) gene plays a role in meat growth and has been shown to increase the growth rate and carcass composition after being given GH. For this function, this gene is used as a strong candidate for genetic markers for meat growth traits. The research objective was to map the growth hormone (GH) gene of the bodyweight of Krui cattle in the Pesisir Barat Regency. Materials and Methods: This research used 30 blood samples of 30 Krui cattle. The method used was by taking quantitative data and blood samples from adult Krui cattle in Pesisir Barat Regency and then the blood samples were analyzed by DNA isolation method. PCR amplification used was a pair of GH-Forward primers: 5 'ATC CAC ACC CCC TCC ACA CAGT 3' and GH-reverse: 5 'CAT TTT CCA CCC TCC CCT ACA G 3', as well as digestion using the RFLP method at the Laboratory of Animal Breeding and Genetics of Universitas Gadjah Mada, Yogyakarta. Association between genotype and body weight was analyzed descriptively. Results: The results showed that Krui cattle had polymorphic genes with three genotypes found, namely: CC, CT and TT. Cattle with CT genotype had the largest average body weight or meat production compared to those with other genotypes. Conclusion: These results indicated that the GH gene identifier has strong evidence that it can be used as a selection tool with the help of genotypes of body weight traits of Krui meat production in the Pesisir Barat Regency. Krui cattle with CT genotype can be developed further because it has high economic value with high average body weight and meat production.