Over the past few years, biogenic methods for designing silver nanocomposites are in limelight due to their ability to generate semi-healthcare and para-pharmaceutical consumer goods. The present study reports the eco-friendly synthesis of silver nanoparticles from the hitherto unexplored mucus of territorial snail Achatina fulica by the facile, clean and easily scalable method. The detailed characterization of the resultant samples by UV–Visible Spectroscopy, FESEM-EDS, XRD and FTIR Spectroscopy techniques corroborated the formation of silver nanoparticles in snail mucus matrix. The resultant samples were tested against a broad range of Gram positive and Gram negative bacteria like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and a fungal strain Aspergillus fumigatus by well diffusion method. The results indicate that silver nanoparticles in mucus matrix exhibit strong antibacterial as well as antifungal activity. The pertinent experiments were also performed to determine the inhibitory concentration against both bacterial and fungal strains. Anticancer activity was executed by in vitro method using cervical cancer cell lines. Curiously, our biogenically synthesized Ag nanoparticles in biocompatible mucus revealed anticancer activity and demonstrated more than 15% inhibition of Hela cells. We suggest an interesting possibility of formulating antimicrobial and possibly anticancer creams/gels for topical applications in skin ailments.

Herein, the preparation of gold nanoparticles-silk fibroin (SF-AuNPs) dispersion and its label-free colorimetric detection of the organophosphate pesticide, namely chlorpyrifos, at ppb level are reported. The silk fibroin solution was extracted from B. mori silk after performing degumming, dissolving and dialysis steps. This fibroin solution was used for synthesis of gold nanoparticles in-situ without using any external reducing and capping agent. X-ray Diffractometry (XRD), Field Emission Transmission Electron Microscopy (FETEM) along with Surface Plasmon Resonance based optical evaluation confirmed generation of gold nanoparticles within SF matrix. The resultant SF-AuNPs dispersion exhibited rapid and excellent colorimetric pesticide sensing response even at 10 ppb concentration. Effect of additional parameters viz. pH, ionic concentration and interference from other pesticide samples was also studied. Notably, SF-AuNPs dispersion exhibited selective colorimetric pesticide sensing response which can be calibrated. Furthermore, this method was extended to various simulated real life samples such as tap water, soil and agricultural products including plant residues to successfully detect the presence of chlorpyrifos pesticide. The proposed colorimetric sensor system is facile yet effective and can be employed by novice rural population and expert researchers alike. It can be exploited as preliminary tool for label-free colorimetric chlorpyrifos pesticide sensing in water and agricultural products. Pesticides act as toxic compounds to all living organisms and hence used to control the insects, weeds, nematodes and other various pests. The pesticides can be carcinogenic in nature, which can produce disorders in bone marrow and nervous system and can sometimes cause infertility and respiratory diseases. Organophosphates (OPs) are among the major pesticides that are largely used in agriculture owing to their relatively low persistence and high effectiveness in insect and pest eradication 1. They are synthetic esters, amides, or thiol derivatives of the phosphoric, phosphonic, phosphorothioic, or phosphonothioic acids 2,3 and are used all over the world to control agricultural, household and structural pests. They are usually considered as safe for agricultural applications due to their relatively faster degradation rates 4. Over the past several years, organophosphate turned out to be an important parameter for water quality evaluation 5. Pesticides in residual form can enter into humans through the food chain (fishes, raw vegetables, fruits and water) 6. As insecticidal mechanism, OPs inhibit acetylcholine-esterase (AChE) activity in insects providing the pesticide action 4,7,8. However, they can also affect the nervous system of other organisms as well as humans imparting toxicity and number of environmental and health issues associated with them have not only provoked the public concern but also research activities for

A simple chemical reduction method was employed to synthesize Cu-Ag and Ag-Cu core-shell nanostructures inside polyvinyl alcohol (PVA) matrix at room temperature. The core-shell nanostructures have been synthesized by varying the two different concentrations (i.e. 0.1 and 0.01 M) of the respective metal ions in equimolar ratios using successive reduction with hydrazine hydrate (HH) as a reducing agent. The core-shell nanostructures have been further characterized by different characterization techniques. The UV-visible spectroscopy exhibit the respective shift in the band positions suggesting the formation of core-shell nanostructures, which was further confirmed by field emission transmission electron microscopy-high-angle-annular dark field elemental mapping.The effect of metal ion concentration of the core-shell nanostructure on various Gram positive and Gram negative bacteria like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and one fungal species Aspergillus fumigatus was observed by performing MIC and MBC/MFC study. Cu-Ag core-shell nanostructures were found to be effective antibacterial agent against all tested Gram-positive and Gram-negative bacteria, whereas Ag-Cu core-shell nanostructures were more efficient against a particular fungal species known as A. fumigatus. The highest value of MIC (75 µg ml −1 ) for Ag-Cu 0.1M core shell nanostructures (D1) was noted against S. aureus and E. coli whereas the lowest value (20 µg ml −1 ) was observed with P. aeruginosa. While in case of Cu-Ag 0.1M core shell nanostructures (E1) the highest value of MIC (100 µg ml −1 ) was noted against S. aureus and P. aeruginosa whereas the lowest value (15 µg ml −1 ) was observed with A. fumigatus.Also, field effect scanning electron microscope (FESEM) images of untreated and core-shell nanoparticles treated micro-organisms showed that 0.1 M Ag-Cu and 0.1 M Cu-Ag core-shell nanostructure can successfully break the cell wall of the fungi A. fumigatus and bacteria P. aeruginosa, respectively. Thus the present study concludes that, Cu-Ag & Ag-Cu core-shell nanostructures damage the cell structure of micro-organisms and inhibits their growth. Hence, the present Cu-Ag & Ag-Cu core-shell nanostructure acts as good antimicrobial agent against the bacteria and fungi, respectively.

The herpes simplex virus type 1 (HSV-1) capsid protein VP24 (encoded by UL26) was expressed as a GST-fusion protein and used to prepare a group of monoclonal antibodies. These were used to characterize the protein in capsids and virus infected cells and demonstrated that it exists as two polypeptide species. The nature of the relationship between these two species was investigated and found to be associated with disulphide bonding. Under non-reducing conditions a species corresponding to dimers of VP24 was identified in preparations of B capsids, the site of action of the proteinase. Biochemical subcellular fractionation studies suggested that only cleaved forms of UL26 and UL26.5 gene products could be detected in the nucleus of the infected cell at early times postinfection.

Dialyzed natural polymer, fibroin, from Bombyx mori was used to synthesize biocompatible silver and gold nanoparticles in-situ in dispersion form. The films of pure fibroin (PF), fibroin-silver nanocomposite (FSNC) and fibroin-gold nanocomposite (FGNC) were fabricated by drop casting method. The characterization of the resultant dispersion and films was performed by visual color change, UV-Vis spectroscopy and atomic force microscopy. The dispersions of PF, FSNC and FGNC were tested for antibacterial activity against E. coli NCIM 2065, S. aureus NCIM 5021, K. pneumoniae NCIM 2957, P. aeruginosa ATCC 9027 and antifungal activity against A. fumigatus NCIM 902. FSNC dispersion exhibited an effective antimicrobial action against all the tested microbes as compared to FGNC dispersion. The mechanism of action for FSNC and FGNC against these microorganisms is proposed. Additionally, the larvicidal activity of the films was investigated against the larvae of Aedes aegypti. The films of FSNC exhibited 100% mortality while the films of FGNC revealed 86–98% mortality against all the larval instars and pupae of A. aegypti. The phytotoxicity study of the nanocomposite films was also carried out to confirm the reusability of water. This is first noble metal nanocomposite based report on larvicidal activity of zika virus vector.