The statistics for divorce in the United States are alarming. Among the shouting there are voices that are not being heard: the children’s. Empirical research has shown that the implementation of protective measures may increase the probability of a child becoming resilient in the face of divorce. This review of current literature is written to discuss what resiliency is and what protective factors will benefit the child. Counseling implications will be presented also.

ABSTRACT:A series of stable breast cancer resistance protein (BCRP, ABCG2) knockdown cell lines were produced by transduction of Caco-2 cells with lentiviral vector-based short hairpin RNA (shRNA). Caco-2 cell is a human intestinal-derived cell line widely used to study intestinal drug absorption. Caco-2 expresses three apical drug efflux transporters: BCRP, P-glycoprotein (P-gp; ABCB1), and multidrug resistance protein 2 (MRP2, ABCC2). BCRP and P-gp in particular play a significant role in pharmacokinetics because of their expression at several key interfaces. Overexpression of BCRP in cancer cells may also be a mechanism of tumor resistance to chemotherapeutic drugs. The goal of this study was to engineer and characterize Caco-2 cell clones with stable knockdown of BCRP expression. The shRNA/BCRP lentiviral particles were used to infect a stable clone of Caco-2 cells. Expression of BCRP was monitored using quantitative polymerase chain reaction (qPCR), Western blotting, immunofluorescence microscopy, and bidirectional transport of probe substrates, estrone-3-sulfate (E3S), and pheophorbide A (PhA). Based on qPCR, expression of BCRP mRNA was knocked down in five clones with a maximum of 97% silencing in clone D. Silencing of BCRP gene expression was maintained for at least 25 passages. Expression of BCRP protein was also reduced significantly. Functionally, BCRP knockdown was reflected in significant reduction of the efflux ratio of E3S and PhA. Clone D in particular should be a useful model for identifying and characterizing P-gp substrates and inhibitors without interference from BCRP and/or MRP2. In addition, it can be used in conjunction with wild-type or vector control Caco-2 cells to identify BCRP substrates.

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This chapter will provide a case study (loosely based on the authors' experiences) that depicts a Black woman, Dr. Jackie, who works in a predominantly white university setting and her experiences with “Karens.” Three different scenarios will be presented within the case study to describe the interactions between the “Karens” and Dr. Jackie. The thought process and emotions that Dr. Jackie experiences after each interaction will be described in detail, as she attempts to brainstorm different ways to address the situation. The chapter will culminate with Dr. Jackie's determination to strategize a plan of action for creating a more serene work environment for herself.