The presence of a perforation is known to significantly compromise the outcome of endodontic treatment. One potential use of regenerative endodontic therapy may be the repair of root canal perforations. In addition to nutrients and systemic in-situ interactions, the three main components believed to be essential for tissue regeneration are: stem cells, scaffold, and growth factors. This study investigated the role of each component of the tissue engineering triad in the organization and differentiation of Dental Pulp Stem Cells (DPSCs) in a simulated furcal perforation site using a mouse model. Collagen served as the scaffold and dentin matrix protein 1 (DMP1) was the growth factor. Materials were placed in simulated perforation sites in dentin slices. MTA was the control repair material. At six weeks, the animals were sacrificed and the perforation sites were evaluated by light microscopy and histological staining. Organization of newly derived pulp tissue was seen in the group containing the triad of DPSCs, a collagen scaffold, and DMP1. The other four groups did not demonstrate any apparent tissue organization. Under the conditions of the present study, it may be concluded that the triad of DPSCs, a collagen scaffold, and DMP1 can induce an organized matrix formation similar to that of pulpal tissue, which may lead to hard tissue formation.
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