The prodigiosin was isolated from Serratia marcescens and the production process was optimized by response surface methodology-central composite design (CCD). The bactericidal efficiency of prodigiosin was analyzed against antibiotic resistant pathogens. The maximum yield of prodigiosin (70.4023 g) was achieved per kg of tannery fleshing at optimized condition time, 81.2 h; temperature, 29°C; pH, 6.8; moisture, 50%. The prodigiosin exhibited complete inhibition of the growth of Pseudomonas aeruginosa compared with other conventional antibiotics which is confirmed by disc diffusion method, LIVE/DEAD assay and fluorescence emission image. Prodigiosin, the bioactive compound was produced from tannery solid waste in solid state fermentation. The antimicrobial effect prodigiosin antibiotic resistant pathogens may make a possible platform in future for bacterial disinfection in clinical/ pharmaceutical wastewater.