Bovine respiratory disease caused by Mycoplasma bovis is a major health problem of cattle worldwide. It inflicts considerable financial losses on beef herds and is the most common cause of mortality in dairy cattle. Bacteriological examination of 35 nasal cavity samples from calves younger than three months of age identified Mycoplasma bovis in eight (22.9%) samples. These cattle were followed until 17 months of age, and repeated examination of nasal cavity samples before necropsy identified Mycoplasma bovis in four (11.4%) samples. At necropsy and lung samples for bacteriological and histological examination were collected. To identify microorganisms from the Mollicutes class isolated from the nasal cavities of cattle we used the PCR method. Furthermore, Mycoplasma bovis was identified on the grounds of biochemical characteristics and by the disk growth inhibition test. The organism was found in 5.7% of calves younger than three months of age in combination with Pasteurella spp. Mycoplasma bovis in combination with Pasteurella multocida and Mannheimia haemolytica was isolated from 5.7% and 2.9% of cattle at 17 months. However, Pasteurella multocida was common in cattle at 17 months and Mannheimia haemolytica was isolated from both age groups of cattle. Histopathological examination of lung samples revealed broncho-interstitial pneumonia in 14.3% of samples. Mycoplasma bovis was isolated from 60.0% of broncho-insterstitial pneumonia cases. The organism was isolated more frequently from the group of calves rather than from the cattle group (P < 0.05). The most common bacterial agents were Pasteurella multocida and Mannheimia haemolytica.

The aim of this study was to make a survey of the presence of Mycoplasma dispar on a cattle breeding farm and to determine antimicrobial susceptibility of the isolates.The study was carried out at a farm in Lithuania. Nasal swabs for bacteriological investigation were collected from ninety dairy, beef and mixed type of cattle from 90 to 300 days of age. Mycoplasma cultivation procedures were carried out using Friis selective media. To confirm the presence of Mollicutes class the polymerase chain reaction (PCR) was used. Isolates were identified according to biochemical and antigenic characteristics.The minimum inhibitory concentration of twenty field isolates of Mycoplasma dispar to tulathromycin, tylosin, lincomycin, enrofloxacin, florfenicol, and oxytetracycline was determined by using a micro-broth dilution method.Mycoplasma dispar was detected in the nasal cavity of 15 out of 84 clinically healthy animals (17.9 %), and in 5 out of 6 animals with respiratory disorders (83.3 %). The isolates were most susceptible to tulathromycin, lincomycin, enrofloxacin and florfenicol. Three (15 %) isolates were resistant to oxytetracycline.The susceptibility to oxytetracycline significantly differed between Mycoplasma dispar isolates compared to the susceptibility of tulathromycin (P < 0.001), lincomycin (P < 0.001) tylosin (P < 0.001), enrofloxacin (P < 0.001), and florfenicol (P < 0.001).