In this study, the temperature effect on the aqueous two-phase systems composed of isopropanol + trisodium citrate + water was studied by experimental measurement of the binodal curves and tie-line compositions at T = 288.15, 298.15, 308.15, and 318.15 K temperatures. It was found that the salting-out ability of the system was increased with increasing the temperature so that the extension of the two-phase area, especially in the salt-rich region, was increased by raising the temperature. Correlation of the tie-line compositions to a Seteschenow-type equation also showed that the two-phase formation tendency of the studied systems has a positive correlation with the salting-out parameters, K ca , of this equation so that K ca values were increased with increasing the temperature. Binodal curves at all studied temperatures were successfully fitted to two temperature-dependent equations with only three fitting parameters, simultaneously. Also, the electrolyte-Wilson model was modified as a function of temperature and used for simultaneous correlation of the tie-line data at all studied temperatures. An excellent agreement between the calculated values of the modified electrolyte-Wilson model and the experimental results was obtained.