The purpose of this paper is to identify middle-troposphere circulation patterns associated with high ozone concentrations during June-August of 2000-07 in the Atlanta, Georgia, metropolitan statistical area (MSA), which is located in the southeastern United States. The methods involved classifying daily 500-hPa geopotential height fields into synoptic types, determining the mean atmospheric conditions (i.e., daytime temperature, daytime relative humidity, daytime cloud cover, morning mixing height, and afternoon mixing height) for the types, determining the mean daily maximum 8-h average ozone concentrations for the types, and performing back-trajectory analyses for high-ozone types. There were a total of 12 synoptic types, and significantly high ozone concentrations across the MSA coincided with the three following types: Atlanta under a continental anticyclone, Atlanta to the east of a continental anticyclone and west of a trough, and Atlanta under the western side of a trough. The continental-anticyclone type was much more prevalent than the other two types. When the MSA was under or just to the east of a continental anticyclone, atmospheric conditions were conducive to increased in situ ozone production and pollutant carryover from the previous day. Between 45% and 60% of the days with those circulation patterns had ozone concentrations exceeding the federal standard. When the Atlanta MSA was under the western side of a trough, not only did the potential for in situ ozone production and pollutant carryover contribute to high ozone concentrations, but there also was a high potential for pollutant transport from the Ohio River valley into the Atlanta MSA.

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The frequent occurrence of exceptionally very heavy rainfall in Mexico during the summer causes flash floods in many areas and major economic losses. As a consequence, a significant part of the annual government budget is diverted to the reconstruction of the disasters caused by floods every year, resulting hold up in the country development. A key element to mitigate the flash flood hazards is the implementation of an early warning system with the ability to process the necessary information in the shortest possible time, in order to increase structural and non-structural resilience in flood prone regions. The real-time estimation of rainfall is essential for the implementation of such systems and the use of remote sensing instruments that feed the operational rainfall-runoff hydrological models is becoming of increasing importance worldwide. However, in some countries such as Mexico, the application of such technology for operational purposes is still in its infancy. Here the implementation of an operational hydrological model is described for the Mixcoac river basin as part of the non-structural measures that can be applied for intense precipitation events. The main goal is to examine the feasibility of the use of remote sensing instruments and establish a methodology to predict the runoff in real time in urban river basins with complex topography, to increase the resilience of the areas affected by annual floods. The study takes data from weather radar operated by the National Meteorological Service of Mexico, as input to a distributed hydrological model. The distributed unit hydrograph model methodology is used in order to assess its feasibility in urban experimental basin. The basic concepts underlying the model, as well as calibration and validation are discussed. The results demonstrate the feasibility of using weather radar data for modeling rainfall-runoff process with distributed parameter models for urban watersheds. A product resulting from this study was the development of software Runoff Forecast Model (ASM), for application in distributed hydrological models with rainfall data in real time in watersheds with complex terrain, which are usually found in Mexico.

Poxviruses are complex dsDNA viruses with over 200 genes, many of them with unknown role in the stimulation of immune responses. Among these, the vaccinia virus (VACV) L3L ORF encodes an essential protein for the transcription of the VACV early genes. To the best of our knowledge, the immune response elicited by L3 has not been characterized. In this regard, our data describes a DNA L3-coding plasmid (pL3L) that stimulates both, humoral- and cell-mediated immune responses in a mouse model. Cell-mediated immune responses were measured by IFN-γ and IL-4 ELISPOT assays. We performed CD8+ cells depletion and flow cytometry analysis to account for the contribution of cytotoxic T lymphocytes in the IFN-γ production. Moreover, results from ELISPOT were confirmed by measuring the concentration of IL-4 and IFN-γ in supernatant of antigen-stimulated splenocytes by cytokine ELISA. Additionally, dominant antigenic regions of L3 protein were identified by epitope mapping analysis. Humoral immune responses were assessed by ELISA. Specifically, the production of total IgG, IgG1 (TH-2) and IgG2a (TH-1) were determined one week after the final immunization. Our ELISPOT data shows pL3L-immunized animals to produce significantly higher frequencies of IFN-γ Spot-Forming Cells (SFC) versus controls. IL-4 levels remained unchanged in all three groups, demonstrating the increase in antigen-specific IFN-γ releasing cells. Flow cytometry assay results showed that CD8+ T cells are a major contributor to the production of IFN-γ. Moreover, our formulation enhances the production of total IgG, predominantly IgG2a isotype. Immunization with pL3L promotes a robust cytotoxic immune response, crucial against viral pathogens. In addition, our vaccine candidate promotes an increase in IgG levels, especially IgG2a (TH-1 type). Our data encourages further studies of L3 as a novel antigen in vaccine development against poxviruses.