No abstract

2)where ( A~~~) s D S = X is the area at finite detergent concentration; (Az@)sDs=o is the area at 0 detergent concentration.From Table 111, Fs decreases as SDS increases to 0.5 and then levels off for cut 1. Moreover, the arithmetic products of Fs and total nitrogen (Le., total nitrogen resolubilized) in cut 1 are 11.8, 9.6, 6.4, and 11.7 mg, respectively at 0, 0.2, 0.5, and 1% SDS. Therefore, SDS increases extractability and initial solubility, but decreases resolubility for cut 1 (see Fs, Table 111). Cuts 2 and 3 are completely soluble at all detergent concentrations within experimental error. The solubility results for all cuts agree with qualitative visual observations. CONCLUSIONSThe nitrogenous constituents of freeze-dried Coastal Bermuda grass can be classified as insoluble (RI), extractable-insoluble (RII), extractable-temporarily soluble (SDS extractable cut l), extractable-soluble (cut 1 at 0% SDS, cut 2, and cut 3). These classifications serve as a functional method of determining the end use of forage protein for maximum utilization as food or feedstuff. Cut 1 soluble (NT = 11%); and possibly cuts 2 and 3 (NT 18%) might be made suitable for human use. Cut 1, SDS extractable (NT = 28% at 1% SDS), cut 2 and 3, and possibly RII (NT N 5% at 1% SDS) might serve as a protein source for monogastric animals and RI and RII (NT N 40% at 1% SDS) for ruminants.The degradation or precipitation of cut 1 could be minimized by separating it, before the chloroplastic proteins are solubilized and with the utmost rapidity, from cuts 2 and 3. Here, cut 1 was separated from cuts 2 and 3 in less than 4 h from the start of the extraction. Since NT, percent CP, molecular weight distribution parameters (Table 11), solubility parameters (Table 111), and the content of aromatics (Table 111) are independent of SDS concentration for cuts 2 and 3, a two-stage procedure apparently would be appropriate: stage 1, extract with buffers, antioxidant and mild conditions to separate RI and RII from cuts 1-3; stage 2, extract RI and RII with harsher conditions (e.g., detergent, pressure, heat, organic solvents, etc.) which would separate maximum chloroplastic protein.ACKNOWLEDGMENT sample extractions and analyses.

A simple method employing simultaneous extraction and oxidation has been developed for the semiautomated determination of ascorbic and dehydroascorbic acids in food products. Recovery studies were conducted on ready-to-eat breakfast cereals and both fresh and canned fruits and vegetables, with average recoveries of 101, 100, and 102%, respectively. Reproducibility data were generated showing a relative standard deviation of 3.5%. The automated method was compared with the manual AOAC fluorometric method and with indophenol titration ; correlation coefficients were 0.9960 and 0.9926, respectively. The hydrolysis product of dehydroascorbic acid, 2,3-diketoguIonic acid, a reported interference in this method, was prepared and shown not to form an interfering fluorescent derivative.

OF RIBOFLAVINE structures. Their pharmacological effects are quite different even though the molecules are presumably unchanged in vivo vs. the solid state (Canepa et al., 1966). This implies that the portions which are not chemically and structurally in common are causing the observed variations in the inhibition of AChE. Similarly, there is a strong case for steric involvement in the mechanism of many, if not all, OP insecticides. Consequently, the precise distances afforded by X-ray crystallographic techniques will prove indispensable in the overall study of these processes.

No abstract