Comparative phenotypic, chemotaxonomic and genetic analysis revealed significant similarities among strains of the genera
Tepidiphilus
and
Petrobacter
. Analysis of 16S rRNA gene sequences and DNA–DNA relatedness of the type strains
Tepidiphilus margaritifer
N2-214T and
Petrobacter succinatimandens
4BONT showed sequence similarity of 98.9 % and less than 40 % relatedness, indicating that these strains represent different species of same genus. Both strains had phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol as major polar lipids. Their fatty acid profiles were almost identical, with the predominant fatty acids C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. In view of this, we propose to transfer the member of the genus
Petrobacter
to the genus
Tepidiphilus
as Tepidiphilus succinatimandens comb. nov. and to emend the description of the genus
Tepidiphilus
. Further, a novel bacterium, strain JHK30T, was isolated from a terrestrial hot spring located at Jharkhand, India, and was identified following a polyphasic approach. Cells were non-sporulating, aerobic, Gram-stain-negative rods and motile by a single polar flagellum. Optimum temperature for growth was 50–55 °C at pH 6.5–7.0. 16S rRNA gene sequence analysis revealed 99.71 % similarity with
P. succinatimandens
4BONT ( = DSM 15512T) and 98.71 % with
T. margaritifer
N2-214T ( = DSM 15129T). However, DNA–DNA relatedness of strain JHK30T with these two type strains was well below 70 %. The DNA G+C base composition was 66.1 mol%. Strain JHK30T represents a novel species of the genus
Tepidiphilus
for which the name Tepidiphilus thermophilus sp. nov. is proposed. The type strain is JHK30T ( = JCM 19170T = LMG 27587T= DSM 27220T).
Idiomarina woesei strain W11T was isolated from the Andaman Sea. Heterotrophic growth was observed at 30 to 37°C and pH 7 to 9. It grows in the presence of 0.5 to 12% (wt/vol) NaCl, and optimum growth occurred at 5 to 6% NaCl. The estimated genome is 2.4 Mb and encodes 2,305 proteins.
A novel Gram-negative, aerobic, motile marine bacterium, strain S4-41(T), was isolated from mucus of the coral Acropora digitifera from the Andaman Sea. Heterotrophic growth was observed in 0-25 % NaCl, at 15-45 °C and pH 4.5-9. In phylogenetic trees, strain S4-41(T) was grouped within the genus Salinicola but formed a separate branch distant from a cluster composed of Salinicola salarius M27(T) and Salinicola socius SMB35(T). DNA-DNA relatedness between strain S4-41(T) and these reference strains were well below 70 %. Q-9 was the sole respiratory quinone. The DNA G+C content was determined to be 63.6 mol%. Based on a polyphasic analysis, strain S4-41(T) is concluded to represent a novel species in the genus Salinicola for which the name Salinicola acroporae sp. nov. is proposed. The type strain is S4-41(T) (=JCM 30412(T) = LMG 28587(T)). Comparative 16S rRNA analysis of the genera Salinicola, Kushneria, Chromohalobacter and Cobetia revealed the presence of genus specific sequence signatures. Multilocus sequence analysis based on concatenated sequences of rRNAs (16S and 23S) and four protein coding housekeeping genes (atpA, gyrB, secA, rpoD) was found to be unnecessary for phylogenetic studies of the genus Salinicola.
Tepidiphilus thermophilus strain JHK30T was isolated from a hot spring at Surajkund, Jharkhand, India. It is a Gram-negative rod, nonsporulating, aerobic, and motile. The estimated genome is 2.3 Mb, with 2,186 protein-coding sequences.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.