Taxonomic study of the genus Tepidiphilus: transfer of Petrobacter succinatimandens to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov., emended description of the genus Tepidiphilus and description of Tepidiphilus thermophilus sp. nov., isolated from a terrestrial hot spring

Abstract

Comparative phenotypic, chemotaxonomic and genetic analysis revealed significant similarities among strains of the genera Tepidiphilus and Petrobacter . Analysis of 16S rRNA gene sequences and DNA–DNA relatedness of the type strains Tepidiphilus margaritifer N2-214T and Petrobacter succinatimandens 4BONT showed sequence similarity of 98.9 % and less than 40 % relatedness, indicating that these strains represent different species of same genus. Both strains had phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol as major polar lipids. Their fatty acid profiles were almost identical, with the predominant fatty acids C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. In view of this, we propose to transfer the member of the genus Petrobacter to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov. and to emend the description of the genus Tepidiphilus . Further, a novel bacterium, strain JHK30T, was isolated from a terrestrial hot spring located at Jharkhand, India, and was identified following a polyphasic approach. Cells were non-sporulating, aerobic, Gram-stain-negative rods and motile by a single polar flagellum. Optimum temperature for growth was 50–55 °C at pH 6.5–7.0. 16S rRNA gene sequence analysis revealed 99.71 % similarity with P. succinatimandens 4BONT ( = DSM 15512T) and 98.71 % with T. margaritifer N2-214T ( = DSM 15129T). However, DNA–DNA relatedness of strain JHK30T with these two type strains was well below 70 %. The DNA G+C base composition was 66.1 mol%. Strain JHK30T represents a novel species of the genus Tepidiphilus for which the name Tepidiphilus thermophilus sp. nov. is proposed. The type strain is JHK30T ( = JCM 19170T = LMG 27587T= DSM 27220T).

Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

“…However, Tepidiphilus thermophilus reduced nitrate only to nitrite (44). Thermophilic denitrifiers, which reduce nitrate to nitrite only, are thus not at all rare and include Petrobacter sp.…”

Tepidiphilus olei sp. nov., isolated from the production water of a water-flooded oil reservoir in PR China

“…Although the type species was first reported in 2003, this genus only comprises three species at present: Tepidiphilus margaritifer , Tepidiphilus succinatimandens and Tepidiphilus thermophilus . The isolation of these micro-organisms frequently occur in geothermal conditions, such as thermophilic water treatment sludge [1], petroleum reservoir [2] and a terrestrial hot spring [3], which favour specific metabolic microbial types. This may explain to some degree why such few species in this genus has been discovered.…”

“…thermophilus DSM 27220 T (=JCM 19170 T ). Unless it was indicated otherwise, all strains were grown and maintained as mentioned previously [1–3].…”

Comparative 16S rRNA signatures and multilocus sequence analysis for the genus Salinicola and description of Salinicola acroporae sp. nov., isolated from coral Acropora digitifera

“…Preparation of fatty acid methyl esters, gas chromatographic separation, analysis and identification of peaks were performed by a method described earlier (Kumari et al 2014b). Polar lipids were extracted from 200 mg freeze dried cells following the method described by Bligh and Dyer (1959), separated by two dimensional TLC on silica gel 60 F 254 plate (Merck, Germany) and analyzed by spraying with appropriate solvents (Poddar et al 2014). Solvent systems used in this study were taken from Kumari et al (2014a).…”

“…Amplification, sequencing and secondary structure based analysis of 16S rRNA were performed as described by Poddar et al (2014) where the 16S rRNA of Esherichia coli (accession no J01695) was used as the reference secondary structure template. The primer and PCR conditions for amplification of 16S rRNA were adapted from de la Haba et al (2010a).…”