Bio-SRF is a biofertilizer formulation containing a mixture of several types of microbes improving soil fertility. The aims of this study were to assess the production process of Bio-SRF biofertilizer and to identify its effectivity on Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4  10 7 cfu/g, Lactobacillus sp. 3,8  10 7 cfu/g, Burkholderia seminalis 7,4  10 8 cfu/g, Pseudomonas stutzeri 4,5  10 8 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m 2 , berat kering umbi 3,27 kg/m 2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah

Halotolerant bacteria are reported as a potential biostimulant to mitigate saline stress on various crops. The mechanism of halotolerant bacteria in elevating plant growth under saline stress is associated with their plant growth-promoting (PGP) traits. This study evaluated the activity of single-strain halotolerant phosphate-solubilizing bacteria and its consortia with halotolerant bacteria that were able to fix nitrogen and produce ACC deaminase in alleviating salinity stress on Zea mays seedlings under in vitro conditions. One single strain and five consortia of bacterial isolates were tested for seedling growth assay under four salinity levels (0, 60, 80, and 100 mM). In vitro assay showed that halotolerant bacteria B1 (Vibrio alginolycticus) and all consortia significantly increased root number at 60 mM salinity level. Consortium B3 (V. alginolyticus+Salinicola zeshunii) was also able to improve the fresh weight of seedlings significantly (by 63.3%). Moreover, inoculation of consortium B3 affected more proline and soluble sugar accumulation in Z. mays seedlings when compared to uninoculated seedlings. We conclude that the application of a consortium of halotolerant bacteria V. alginolyticus+S. zeshunii was potentially used in improving Z. mays growth in slightly and moderately saline areas.