This study aims to observe the relationship between leaves' relative water content (RWC), soluble sugar, dry matter accumulation, and rice yield components under water-stress conditions during the generative stage. Six rice varieties were observed under three different water stress treatments: non-stress, moderate water-stress (-0.35 bar), and severe (-0.70 bar). The results showed that under moderate and severe water-stress; the RWC at reproductive stage had correlation with RWC at maturity stage (r = 0.97), colored pollen (r = 0.87), the percentage of grains (r = 0.54) and grain weight (r = 0.57). The RWC at anthesis stage was closely related to the RWC at ripening stage (r = 0.99), the percentage of colored pollen (r = 0.87) and filled grains (r = 0.58), and the weight of grains per hill (r = 0.69). The RWC at the ripening stage was also closely related to the percentage of colored pollen (r = 0.87), the percentage of filled grains (r = 0.61), and the weight of filled grains per hill (r = 0.72). Furthermore, the percentage of colored pollen was closely related to the biomass dry weight (r = 0.47), and the soluble sugars at the reproductive stage were related to the percentage of filled grains (r = -0.68). The soluble sugars at the anthesis stage were closely related to the soluble sugars at the maturity stage (r = 0.75). The result might allow us to use the RWC as a determinant for rice under water stress, and it is important to maintain the RWC under water stress, especially during the generative stage.