Purpose: To verify the copaiba balsam (Copaifera officinalis) effect on Walker 256 carcinoma inoculated into vagina and uterine cervix of rats. Methods: Eighteen female Wistar rats weighing between 180-250g were used, distributed into 2 groups (GCop, GC). On the 1st day of the experiment, 0.3 ml of Walker 256 carcinoma (2x10 6 concentration) was inoculated in both groups; on the 3rd day of the experiment, it was given 4.8 ml/kg of distilled water to the GC group, and 4.8 ml/kg of copaiba balsam to the GCop group. On the 12th day, euthanasia was performed and the tumor was grafted, being weighted and verified its volume. The data were submitted to statistical analysis with ANOVA test. Results: It was observed that copaiba balsam presented a negative inhibitory potential of 70%. Conclusion:The copaiba balsam stimulated the tumor growth. Key words: Plants, Medicinal. Carcinoma 256, Walker. Uterine Cervical Neoplasms. Rats. RESUMOObjetivo: Verificar o efeito do óleo de copaíba da espécie Copaifera officinalis no carcinoma de Walker 256 inoculado em vagina e colo de útero de ratas. Métodos: Foram utilizadas 18 ratas da linhagem Wistar, pesando entre 180-250g, distribuídas em dois grupos (CCop, GC). No 1º dia de experimento, em ambos os grupos foi inoculado 0,3ml de tumor de Walker 256 na concentração de 2x10 6 ; no 3º dia após essa inoculação, foi iniciada a administração de água destilada na dose de 4,8 ml/kg ao GC, e copaíba na dose de 4,8 ml/kg ao GCop. No 12° dia foi realizada a eutanásia das ratas e ressecado o tumor, sendo este pesado e averiguado seu volume. Os dados obtidos foram submetidos à análise estatística pelo método ANOVA. Resultados: Observou-se que o óleo de copaíba apresentou um potencial inibitório negativo de 70%. Conclusão: O óleo de copaíba estimulou o crescimento tumoral. Descritores: Plantas Medicinais. Carcinoma 256 de Walker. Neoplasias do Colo do Útero. Ratos.

Purpose: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. Methods: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. Results: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. Conclusion: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass. Keywords: Ovariectomy. Cervix Uteri. Vagina. Carcinoma 256, Walker. Rats. RESUMOObjetivo: Verificar o efeito da ooforectomia à inoculação do tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 10 ratas Wistar, fêmeas, virgens, adultas, distribuídas em dois grupos de estudo com 05 animais cada: grupo tumor (GT): ratas inoculadas com tumor de Walker 256, e grupo Ooforectomia (GO): ratas ooforectomizadas e inoculadas com tumor de Walker 256. No dia anterior à inoculação vaginal do tumor, foram inoculados 0,3ml de ácido acético na vagina das ratas de ambos os grupos; no dia seguinte, foi realizada a escarificação da parede vaginal com uma escova de endocérvice e inoculado tumor de Walker 256. Após 12 dias, foi removido o tumor em bloco com vagina e cornos uterinos para análise macro e microscópica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença estatística entre os dois grupos; no entanto, notou-se que o comportamento de crescimento do tumor no grupo GO apresentou maior invasividade em extensão, comprometendo cornos uterinos. Conclusão: Observou-se que o grupo GO apresentou comportamento macroscópico diferente ao grupo GT, no entanto, em termos totais de massa tumoral ambos apresentam desenvolvimento similar. Descritores: Ovariectomia. Colo do Útero. Vagina. Carcinoma 256 Walker. Rats.

Purpose:To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. Methods: Fifteen female rats were used, and assigned to three groups each one with fi ve rats: group A -rats with 4x10 6 cells of Walker 256 carcinoma without acid acetic inoculation; group B -rats with 2x10 6 cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10 6 cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance -ANOVA. P values < 0.05 were taken to indicate statistical signifi cance. Results: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. Conclusion: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10 6 or 4x10 6 cells on its inoculation. Key words: Carcinoma 256, Walker. Cervix uteri. Vagina. Disease models, animal. Rats. RESUMOObjetivo: Estabelecer um modelo de inoculação de Tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 15 ratas fêmeas, virgens, adultas, pesando entre 200-250g, distribuídas em três grupos de estudo com cinco animais cada: grupo A (GA): ratas com tumor de Walker 256 em concentração de 4x10 6 sem ácido acético; grupo B (GB): ratas com tumor de Walker 256 em concentração de 2x10 6 células com ácido acético; grupo C (GC): ratas com tumor de Walker 256 em concentração de 4x10 6 células com ácido acético. No dia anterior à inoculação do tumor, foi realizada a inoculação de 0,3 ml de ácido acético a 10% na vagina das ratas de GB e GC; no dia seguinte, tanto estas como as ratas do grupo GA foram anestesiadas, feita a escarifi cação da parede vaginal com uma escova de endocérvice e inoculado 0,3ml de tumor na concentração de 4x10 6 células nos grupos GA e GC e 2x10 6 células no grupo GB. Após 12 dias, foi realizada a eutanásia e removido o tumor em bloco com vagina e cornos uterinos para análise, sendo pesado e averiguado seu volume e calculado as relaçõe...