This research aimed to study the antibacterial activity and Antibiotic effect of hot aqueous extract of leaves of Ziziphus spina Christy (Sidr),against clinical isolate of Staphylococcus wernari. four isolates were initially diagnosed as Staphylococci bacteria, then one isolation was chosen depending on its sensitivity  and resistance to several different types of antibiotics. The VITEK-2 compact system (ID and AST) and PCR were used to confirm the type of staphylococcal isolates . The results showed that one isolate was due to Staphylococcus wernari.  the ability of isolate to form biofilm was tested using the Microtiter plate method (96 wells). The results indicated that the extracted isolate was able to produce a biofilm. Where there was a clear effect of moxifloxacin and penicillin G with (MIC), (Sub-MIC) and (Sub-MIC) in inhibiting the growth of bacteria, A significant (P < 0.001).Synergistic effect between Penicillin G, Moxifloxacin with (MIC), (Sub-MIC) and (Sub-MIC) were tested with hot aqueous extract of Ziziphus spina christi (Sidr) leaves with 50 mg/mL. The results showed a high synergy between two types of antibiotics and plant extracts.

Background: Helicobacter pylori, a member of Epsilonproteobacteria, is a Gram-negative microaerophilic bacterium that colonizes gastric mucosa of about 50% of the human population. Although most infections caused by H. pylori are asymptomatic, the microorganism is strongly associated with serious diseases of the upper gastrointestinal tract such as chronic gastritis, peptic ulcer, duodenal ulcer, and gastric cancer, and it is classified as a group I carcinogen. The prevalence of H. pylori infections varies worldwide. prevalence among middle-aged adults is over 80 percent in many developing countries, as compared with 20 to 50 percent in industrialized countries. The infection is acquired by oral ingestion of the bacterium and is mainly transmitted within families in early childhood, Humans can also become infected with Helicobacter heilmannii, a spiral bacterium found in dogs, cats, pigs, and nonhuman primates. Conclusion: H. pylori infection stimulates the reaction of autoantibodies with gastric epithelial cells, and this leads to gastritis. These autoantibodies can be directly induced to epithelial cells by activating complement, inducing apoptosis or provoking an antibody-dependent cytotoxic reaction resulting in subsequent tissue destruction.

This study was conducted to detect genotypes and the spread of infection with viral hepatitis B type among children of both sexes (from newborns until the age of 16 years) in four provinces of the middle Euphrates provinces (Najaf, Karbala, Babel, and Diwaniya), 422 samples were collected from seven specialized hospitals (one hospital in Najaf and two hospitals from each of the three remaining provinces). There were 76 samples from children with thalassemia and the other 346 samples were random samples from children. The following technologies, ELIZA (HBsAb, HBsAg) and biochemical measurements of AST and ALT enzyme levels were applied. The percentage of cases of total viral hepatitis B that showed positive results for ELIZA HBsAb test were 8.767 % from total samples, and for ELIZA HBsAg tests, were 0.01 % from total samples.It was found that 15 out of 43 patients show elevation of ALT enzyme or AST enzyme or both enzymes over the normal value. All thalassemic patients show elevation in both ALT enzyme and AST enzyme.