No abstract

No abstract

We report a spectrophotometric method for determining theophylline that is useful as a routine method for monitoring serum theophylline concentrations. The recovery of theophylline from serum is essentially 100% whem compared with aqueous standards taken through the entire procedure. Barbiturates, which interfere with most ultraviolet spectrometric methods for theophylline, do not interfere with this method. The procedure requires less than 45 min for completion and is more sensitive than most spectrophotometric methods for determining theophylline.

This work has demonstrated that when choline is added to the perfusate of isolated livers, these take up the base throughout the duration of the experiment. A sensitive method is described, in which 1,2-14C-choline is used, for estimating the uptake of choline by the liver.