SummaryA post-column chemiluminescent technique for the detection of compounds that are poor chromophores using electrogenerated chemiluminescence following separation by capillary electrophoresis is described. The luminescent signal is generated following the reaction of analytes (e.g. amines) with Ru(bpy)s3+, which is electrochemically generated post-column from Ru(hpyh2+. Tripropylamine and proline are used as two model compounds to demonstrate the feasibility of the method. Detection limits for the prototype system were in the micromolar range, suggesting that this technique offers an alternative to indirect detection of compounds that are poor chromophores with an added selectivity advantage. The system includes the use of a conductive joint to isolate the separation field from the potential necessary to drive the electrogenerated chemiluminescent reaction. Addition of the chemiluminescent reagent Ru(hpy)?+ post-column did not decrease the efficiency of the separation. The design and fabrication of the novel cell is discussed.

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A sensttive Iaser-lnduced ftluorwcence (LIF) detection s c m e for natlve nuclelc acids and DNA restriction fragments separhted by caplllary ektrophoresk (CE) has been developed. The 275.4-nm Hne from an argon Ion laser or the 248nm line from a waveguide KrF laser lo used to excite natlve fluorescence. Detection llmits for guanosine and adenodno monophosphate (1.1 X lo4 and 5 X lo4 M, roapectlvely) are up to 3 orders of magnitude lower than UV dotedon. SenslMyfor natlve Iluorescence of DNA restriction fragmonts In gel-flllod caplllarleo rivals that of UV absorption.The decrease In perlormance In geWHkd separatlons wing LIF detection k caused by the high background associated with gel fluorescence, as well as gel quenching of the fluorescence emkrlon. The development of gels exhlbitlng lower background fluorescence or off -column coupling should lead to signi?lcant Improvements In senrHlvlty over UV detection. Thk novel and practical system enables, for tho flrrt time, the sensltlve detectlon of nuclelc-acld-contalnlng compounds without tho need for fluorescence labeling.

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