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Miniaturization of ligand binding assays may reduce costs by decreasing reagent consumption, but it is less apparent that miniaturized assays can simultaneously exceed the sensitivity of macroscopic techniques by analyte “harvesting” to exploit the total analyte mass available in a sample. Capture reagents (avidin or antibodies) immobilized in 200-μm diameter zones are shown to substantially deplete analyte from a liquid sample during a 1–3-h incubation, and the assays that result sense the total analyte mass in a sample rather than its concentration. Detection of as few as 105 molecules of analyte per zone is possible by fluorescence imaging in situ on the solid phase using a near-infrared dye label. Single and multianalyte mass-sensing sandwich array assays of the IgG subclasses show the sensitivity and specificity of ELISA methods but use less than 1/100 the capture antibody required by the 96-well plate format.

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Polarized infrared spectra of a single crystal of cyclohexane solid II and the infrared and laser Raman of polycrystalline solid II at 90 0 K have been measured. The observed splittings and the infrared polarization results indicate a unique site and factor group for this phase. The suggested crystal structure is orthorhombic D2A• The infrared spectrum of a single crystal of the solid I phase has also been recorded and the results are found to agree with previous x-ray and Raman studies. Pressure techniques employing miniaturized instrumentation were used to grow single crystals of solids I and II.

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